TY - JOUR
T1 - Visualization of fluorescent protein expression in whole-mount postimplantation-stage mouse embryos
AU - Behringer, Richard
AU - Gertsenstein, Marina
AU - Nagy, Kristina Vintersten
AU - Nagy, Andras
PY - 2018/3
Y1 - 2018/3
N2 - Fluorescent protein (FP)-expressing transgenic mice are powerful genetic resources for marking both live and fixed cells and tissues. Expression in live embryos requires only dissection and visualization using an appropriate microscope. Fixation can compromise FP activity. A simple tissue-clearing agent called Scale preserves FP activity while rendering the embryo or organ optically transparent.
AB - Fluorescent protein (FP)-expressing transgenic mice are powerful genetic resources for marking both live and fixed cells and tissues. Expression in live embryos requires only dissection and visualization using an appropriate microscope. Fixation can compromise FP activity. A simple tissue-clearing agent called Scale preserves FP activity while rendering the embryo or organ optically transparent.
UR - http://www.scopus.com/inward/record.url?scp=85042856399&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85042856399&partnerID=8YFLogxK
U2 - 10.1101/pdb.prot092759
DO - 10.1101/pdb.prot092759
M3 - Article
C2 - 29496814
AN - SCOPUS:85042856399
SN - 1940-3402
VL - 2018
SP - 171
EP - 172
JO - Cold Spring Harbor protocols
JF - Cold Spring Harbor protocols
IS - 3
ER -