Visualization of fluorescent protein expression in whole-mount postimplantation-stage mouse embryos

Richard Behringer, Marina Gertsenstein, Kristina Vintersten Nagy, Andras Nagy

Research output: Contribution to journalArticlepeer-review

Abstract

Fluorescent protein (FP)-expressing transgenic mice are powerful genetic resources for marking both live and fixed cells and tissues. Expression in live embryos requires only dissection and visualization using an appropriate microscope. Fixation can compromise FP activity. A simple tissue-clearing agent called Scale preserves FP activity while rendering the embryo or organ optically transparent.

Original languageEnglish (US)
Pages (from-to)171-172
Number of pages2
JournalCold Spring Harbor protocols
Volume2018
Issue number3
DOIs
StatePublished - Mar 2018

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology

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