Visualizing the signal transduction pathways in living cells with GFP-based FRET probes

Kazuo Kurokawa, Akiyuki Takaya, Kenta Terai, Aki Fujioka, Michiyuki Matsuda

    Research output: Contribution to journalReview article

    14 Scopus citations

    Abstract

    Visualizing how signals are transmitted within a living cell has long been a goal of molecular biologists, which has now been realized by probes based on the principle of fluorescence resonance energy transfer (FRET). Variants of green fluorescent protein (GFP) enabled the preparation of genetically-encoded FRET probes, and their application has been expanded for use in many areas of biology. The GFP-based FRET probes can be classified as belonging to one of two types, intermolecular and intramolecular FRET probes. The merit of the intermolecular FRET probe lies in the ease of preparation of the probes, whereas the merit of the intramolecular FRET probe lies in the high signal-to-noise ratio. Although these GFP-based probes are powerful tools for the visualization of signal transduction cascades, numerous pitfalls remain associated with this technique. Here, we provide an overview of the GFP-based FRET probes and discuss these issues.

    Original languageEnglish (US)
    Pages (from-to)347-355
    Number of pages9
    JournalActa Histochemica et Cytochemica
    Volume37
    Issue number6
    DOIs
    StatePublished - Dec 1 2004

    Keywords

    • Biotechnology
    • Fluorescence microscopy
    • Fluorescence resonance energy transfer
    • Green fluorescence protein

    ASJC Scopus subject areas

    • Pathology and Forensic Medicine
    • Biochemistry
    • Physiology
    • Histology
    • Cell Biology

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