Volumetric high-frequency Doppler ultrasound enables the assessment of early antiangiogenic therapy effects on tumor xenografts in nude mice

Manfred Jugold, Moritz Palmowski, Jochen Huppert, Eva C. Woenne, Margareta M. Mueller, Wolfhard Semmler, Fabian Kiessling

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The sensitivity of Doppler ultrasound below 10 MHz to assess antiangiogenic therapy effects in tumor xenografts has been shown to be limited. Thus, our aim was to evaluate high-frequency volumetric power-Doppler ultrasound (HF-VPDU) for monitoring antiangiogenic treatments. Squamous cell carcinoma xenografts grown in nude mice were scanned with HF-VPDU at a center frequency of 30 MHz. Images with 200-μm slice thicknesses were recorded and merged into a three-dimensional dataset, of which the relative color pixel density was determined. Animals received either VEGFR2 antibodies or 0.9% NaCl and were examined at days 0, 3 and 6 of treatment. After the last examination, tumors were resected and their vascularization characterized by immunohistology. At day 6, the volumes of treated and untreated tumors were not significantly different yet. In contrast, mean tumor vascularization in treated animals had decreased to 44%, while in the control group it had increased to 152% (P<0.01). In correspondence, vessel density, as determined by CD31 staining, was 0.19±0.10% in treated and 0.92±0.41% in untreated tumors (P<0.01). Additionally, the fraction of mature (SMA-positive) vessels increased under therapy. Thus, HF-VPDU can be considered as an easily applicable and fast method to screen high animal numbers for antiangiogenic therapy effects.

Original languageEnglish (US)
Pages (from-to)753-758
Number of pages6
JournalEuropean Radiology
Volume18
Issue number4
DOIs
StatePublished - Apr 1 2008

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Doppler Ultrasonography
Secondary Prevention
Heterografts
Nude Mice
Neoplasms
Therapeutics
Squamous Cell Carcinoma
Color
Staining and Labeling
Control Groups
Antibodies

Keywords

  • Angiogenesis
  • High frequency
  • Power Doppler
  • Tumor therapy
  • Ultrasound

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

Volumetric high-frequency Doppler ultrasound enables the assessment of early antiangiogenic therapy effects on tumor xenografts in nude mice. / Jugold, Manfred; Palmowski, Moritz; Huppert, Jochen; Woenne, Eva C.; Mueller, Margareta M.; Semmler, Wolfhard; Kiessling, Fabian.

In: European Radiology, Vol. 18, No. 4, 01.04.2008, p. 753-758.

Research output: Contribution to journalArticle

Jugold, Manfred ; Palmowski, Moritz ; Huppert, Jochen ; Woenne, Eva C. ; Mueller, Margareta M. ; Semmler, Wolfhard ; Kiessling, Fabian. / Volumetric high-frequency Doppler ultrasound enables the assessment of early antiangiogenic therapy effects on tumor xenografts in nude mice. In: European Radiology. 2008 ; Vol. 18, No. 4. pp. 753-758.
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