Wide-field and high-resolution optical imaging for early detection of oral neoplasia

Mark C. Pierce, Richard A. Schwarz, Kelsey Rosbach, Darren Roblyer, Tim Muldoon, Michelle D. Williams, Adel K. El-Naggar, Ann M. Gillenwater, Rebecca Richards-Kortum

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Current procedures for oral cancer screening typically involve visual inspection of the entire tissue surface at risk under white light illumination. However, pre-cancerous lesions can be difficult to distinguish from many benign conditions when viewed under these conditions. We have developed wide-field (macroscopic) imaging system which additionally images in cross-polarized white light, narrowband reflectance, and fluorescence imaging modes to reduce specular glare, enhance vascular contrast, and detect disease-related alterations in tissue autofluorescence. We have also developed a portable system to enable high-resolution (microscopic) evaluation of cellular features within the oral mucosa in situ. This system is a wide-field epi-fluorescence microscope coupled to a 1 mm diameter, flexible fiber-optic imaging bundle. Proflavine solution was used to specifically label cell nuclei, enabling the characteristic differences in N/C ratio and nuclear distribution between normal, dysplastic, and cancerous oral mucosa to be quantified. This paper discusses the technical design and performance characteristics of these complementary imaging systems. We will also present data from ongoing clinical studies aimed at evaluating diagnostic performance of these systems for detection of oral neoplasia.

Original languageEnglish (US)
Title of host publicationPhotonic Therapeutics and Diagnostics VI
DOIs
StatePublished - 2010
EventPhotonic Therapeutics and Diagnostics VI - San Francisco, CA, United States
Duration: Jan 23 2010Jan 25 2010

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7548
ISSN (Print)1605-7422

Other

OtherPhotonic Therapeutics and Diagnostics VI
Country/TerritoryUnited States
CitySan Francisco, CA
Period1/23/101/25/10

Keywords

  • Optical imaging
  • cancer screening
  • diagnostics
  • spectroscopy

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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