TY - GEN
T1 - Wide-field and high-resolution optical imaging for early detection of oral neoplasia
AU - Pierce, Mark C.
AU - Schwarz, Richard A.
AU - Rosbach, Kelsey
AU - Roblyer, Darren
AU - Muldoon, Tim
AU - Williams, Michelle D.
AU - El-Naggar, Adel K.
AU - Gillenwater, Ann M.
AU - Richards-Kortum, Rebecca
N1 - Copyright:
Copyright 2011 Elsevier B.V., All rights reserved.
PY - 2010
Y1 - 2010
N2 - Current procedures for oral cancer screening typically involve visual inspection of the entire tissue surface at risk under white light illumination. However, pre-cancerous lesions can be difficult to distinguish from many benign conditions when viewed under these conditions. We have developed wide-field (macroscopic) imaging system which additionally images in cross-polarized white light, narrowband reflectance, and fluorescence imaging modes to reduce specular glare, enhance vascular contrast, and detect disease-related alterations in tissue autofluorescence. We have also developed a portable system to enable high-resolution (microscopic) evaluation of cellular features within the oral mucosa in situ. This system is a wide-field epi-fluorescence microscope coupled to a 1 mm diameter, flexible fiber-optic imaging bundle. Proflavine solution was used to specifically label cell nuclei, enabling the characteristic differences in N/C ratio and nuclear distribution between normal, dysplastic, and cancerous oral mucosa to be quantified. This paper discusses the technical design and performance characteristics of these complementary imaging systems. We will also present data from ongoing clinical studies aimed at evaluating diagnostic performance of these systems for detection of oral neoplasia.
AB - Current procedures for oral cancer screening typically involve visual inspection of the entire tissue surface at risk under white light illumination. However, pre-cancerous lesions can be difficult to distinguish from many benign conditions when viewed under these conditions. We have developed wide-field (macroscopic) imaging system which additionally images in cross-polarized white light, narrowband reflectance, and fluorescence imaging modes to reduce specular glare, enhance vascular contrast, and detect disease-related alterations in tissue autofluorescence. We have also developed a portable system to enable high-resolution (microscopic) evaluation of cellular features within the oral mucosa in situ. This system is a wide-field epi-fluorescence microscope coupled to a 1 mm diameter, flexible fiber-optic imaging bundle. Proflavine solution was used to specifically label cell nuclei, enabling the characteristic differences in N/C ratio and nuclear distribution between normal, dysplastic, and cancerous oral mucosa to be quantified. This paper discusses the technical design and performance characteristics of these complementary imaging systems. We will also present data from ongoing clinical studies aimed at evaluating diagnostic performance of these systems for detection of oral neoplasia.
KW - Optical imaging
KW - cancer screening
KW - diagnostics
KW - spectroscopy
UR - http://www.scopus.com/inward/record.url?scp=79751519051&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79751519051&partnerID=8YFLogxK
U2 - 10.1117/12.848347
DO - 10.1117/12.848347
M3 - Conference contribution
AN - SCOPUS:79751519051
SN - 9780819479440
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Photonic Therapeutics and Diagnostics VI
T2 - Photonic Therapeutics and Diagnostics VI
Y2 - 23 January 2010 through 25 January 2010
ER -