X ray mediated cross linking of protein and DNA

B. D. Minsky, A. Braun

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Using a simple filter assay for the binding of BSA or lysozyme to DNA, two mechanisms of X-ray-mediated cross linking are shown to occur. One, a fast reaction, appears to involve a radical intermediate, is inhibited by high pH and salt, and seems to be enhanced by deoxygenation. The second mechanism, a slow time-dependent component, differs from the fast reaction in its stimulation by histidine, its inhibition by catalase, and the lack of an oxygen effect. Separate irradiation of DNA or water does not lead to cross linking. However, separate irradiation of protein leads to cross linking which proceeds with slow-component kinetics.

Original languageEnglish (US)
Pages (from-to)505-515
Number of pages11
JournalRadiation research
Volume71
Issue number3
DOIs
StatePublished - 1977
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Radiation
  • Radiology Nuclear Medicine and imaging

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