Abstract
Using a simple filter assay for the binding of BSA or lysozyme to DNA, two mechanisms of X-ray-mediated cross linking are shown to occur. One, a fast reaction, appears to involve a radical intermediate, is inhibited by high pH and salt, and seems to be enhanced by deoxygenation. The second mechanism, a slow time-dependent component, differs from the fast reaction in its stimulation by histidine, its inhibition by catalase, and the lack of an oxygen effect. Separate irradiation of DNA or water does not lead to cross linking. However, separate irradiation of protein leads to cross linking which proceeds with slow-component kinetics.
Original language | English (US) |
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Pages (from-to) | 505-515 |
Number of pages | 11 |
Journal | Radiation research |
Volume | 71 |
Issue number | 3 |
DOIs | |
State | Published - 1977 |
Externally published | Yes |
ASJC Scopus subject areas
- Biophysics
- Radiation
- Radiology Nuclear Medicine and imaging