TY - JOUR
T1 - α-Pinene isolated from Schinus terebinthifolius Raddi (Anacardiaceae) induces apoptosis and confers antimetastatic protection in a melanoma model
AU - Matsuo, Alisson L.
AU - Figueiredo, Carlos R.
AU - Arruda, Denise C.
AU - Pereira, Felipe V.
AU - Borin Scutti, Jorge A.
AU - Massaoka, Mariana H.
AU - Travassos, Luiz R.
AU - Sartorelli, Patricia
AU - Lago, João H.G.
N1 - Funding Information:
The present work was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).
PY - 2011/7/29
Y1 - 2011/7/29
N2 - Malignant melanoma is one the most aggressive types of cancer and its incidence has gradually increased in the last years, accounting for about 75% of skin cancer deaths. This poor prognosis results from the tumor resistance to conventional drugs mainly by deregulation of apoptotic pathways. The aim of this work was to investigate the cell death mechanism induced by α-pinene and its therapeutic application. Our results demonstrated that α-pinene was able to induce apoptosis evidenced by early disruption of the mitochondrial potential, production of reactive oxygen species, increase in caspase-3 activity, heterochromatin aggregation, DNA fragmentation and exposure of phosphatidyl serine on the cell surface. Most importantly, this molecule was very effective in the treatment of experimental metastatic melanoma reducing the number of lung tumor nodules. This is the first report on the apoptotic and antimetastatic activity of isolated α-pinene.
AB - Malignant melanoma is one the most aggressive types of cancer and its incidence has gradually increased in the last years, accounting for about 75% of skin cancer deaths. This poor prognosis results from the tumor resistance to conventional drugs mainly by deregulation of apoptotic pathways. The aim of this work was to investigate the cell death mechanism induced by α-pinene and its therapeutic application. Our results demonstrated that α-pinene was able to induce apoptosis evidenced by early disruption of the mitochondrial potential, production of reactive oxygen species, increase in caspase-3 activity, heterochromatin aggregation, DNA fragmentation and exposure of phosphatidyl serine on the cell surface. Most importantly, this molecule was very effective in the treatment of experimental metastatic melanoma reducing the number of lung tumor nodules. This is the first report on the apoptotic and antimetastatic activity of isolated α-pinene.
KW - Apoptosis
KW - Melanoma
KW - α-Pinene
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U2 - 10.1016/j.bbrc.2011.06.176
DO - 10.1016/j.bbrc.2011.06.176
M3 - Article
C2 - 21756878
AN - SCOPUS:79960841845
SN - 0006-291X
VL - 411
SP - 449
EP - 454
JO - Biochemical and biophysical research communications
JF - Biochemical and biophysical research communications
IS - 2
ER -