TY - JOUR
T1 - α5β1 integrin activates an NF-κB-dependent program of gene expression important for angiogenesis and inflammation
AU - Klein, Sharon
AU - De Fougerolles, Antonin R.
AU - Blaikie, Pamela
AU - Khan, Leila
AU - Pepe, Angela
AU - Green, Cynthia D.
AU - Koteliansky, Victor
AU - Giancotti, Filippo G.
PY - 2002
Y1 - 2002
N2 - GeneCalling, a genome-wide method of mRNA profiling, reveals that endothelial cells adhering to fibronectin through the α5β1 integrin, but not to laminin through the α2β1 integrin, undergo a complex program of gene expression. Several of the genes identified are regulated by the NF-κB transcription factor, and many are implicated in the regulation of inflammation and angiogenesis. Adhesion of endothelial cells to fibronectin activates NF-κB through a signaling pathway requiring Ras, phosphatidylinositol 3-kinase, and Rho family proteins, whereas adhesion to laminin has a limited effect. Retroviral transfer of the superrepressor of NF-κB, IκB-2A, blocks basic fibroblast growth factor-induced angiogenesis in vivo. These results suggest that engagement of the α5β1 integrin promotes an NF-κB-dependent program of gene expression that coordinately regulates angiogenesis and inflammation.
AB - GeneCalling, a genome-wide method of mRNA profiling, reveals that endothelial cells adhering to fibronectin through the α5β1 integrin, but not to laminin through the α2β1 integrin, undergo a complex program of gene expression. Several of the genes identified are regulated by the NF-κB transcription factor, and many are implicated in the regulation of inflammation and angiogenesis. Adhesion of endothelial cells to fibronectin activates NF-κB through a signaling pathway requiring Ras, phosphatidylinositol 3-kinase, and Rho family proteins, whereas adhesion to laminin has a limited effect. Retroviral transfer of the superrepressor of NF-κB, IκB-2A, blocks basic fibroblast growth factor-induced angiogenesis in vivo. These results suggest that engagement of the α5β1 integrin promotes an NF-κB-dependent program of gene expression that coordinately regulates angiogenesis and inflammation.
UR - http://www.scopus.com/inward/record.url?scp=0036315845&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036315845&partnerID=8YFLogxK
U2 - 10.1128/MCB.22.16.5912-5922.2002
DO - 10.1128/MCB.22.16.5912-5922.2002
M3 - Article
C2 - 12138201
AN - SCOPUS:0036315845
SN - 0270-7306
VL - 22
SP - 5912
EP - 5922
JO - Molecular and cellular biology
JF - Molecular and cellular biology
IS - 16
ER -