TY - JOUR
T1 - β-Glucan synthesis in the cotton fiber
T2 - III. Identification of UDP-glucose-binding subunits of β-glucan synthases by photoaffinity labeling with [β-32P]5′-N3-UDP-glucose
AU - Li, Likun
AU - Drake, Richard R.
AU - Clement, Sarah
AU - Brown, R. Malcolm
PY - 1993/4
Y1 - 1993/4
N2 - Using differential product entrapment and photolabeling under specifying conditions, we identified a 37-kD polypeptide as the best candidate among the UDP-glucose-binding polypeptides for the catalytic subunit of cotton (Cossypium hirsutum) cellulose synthase. This polypeptide is enriched by entrapment under conditions favoring β-1,4-glucan synthesis, and it is magnesium dependent and sensitive to unlabeled UDP-glucose. A 52-kD polypeptide was identified as the most likely candidate for the catalytic subunit of β-1,3-glucan synthase because this polypeptide is the most abundant protein in the entrapment fraction obtained under conditions favoring β-1,3-glucan synthesis, is coincident with β-1,3-glucan synthase activity, and is calcium dependent. The possible involvement of other polypeptides in the synthesis of β-1,3-glucan is discussed.
AB - Using differential product entrapment and photolabeling under specifying conditions, we identified a 37-kD polypeptide as the best candidate among the UDP-glucose-binding polypeptides for the catalytic subunit of cotton (Cossypium hirsutum) cellulose synthase. This polypeptide is enriched by entrapment under conditions favoring β-1,4-glucan synthesis, and it is magnesium dependent and sensitive to unlabeled UDP-glucose. A 52-kD polypeptide was identified as the most likely candidate for the catalytic subunit of β-1,3-glucan synthase because this polypeptide is the most abundant protein in the entrapment fraction obtained under conditions favoring β-1,3-glucan synthesis, is coincident with β-1,3-glucan synthase activity, and is calcium dependent. The possible involvement of other polypeptides in the synthesis of β-1,3-glucan is discussed.
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U2 - 10.1104/pp.101.4.1149
DO - 10.1104/pp.101.4.1149
M3 - Article
C2 - 12231766
AN - SCOPUS:12044255964
SN - 0032-0889
VL - 101
SP - 1149
EP - 1156
JO - Plant Physiology
JF - Plant Physiology
IS - 4
ER -