12(S)-HETE is a mitogenic factor for microvascular endothelial cells: Its potential role in angiogenesis

Dean G. Tang; Colette Renaud; Sanja Stojakovic; Clement A. Diglio; Arther Porter; Kenneth V. Honn

doi:10.1006/bbrc.1995.1836

12(S)-HETE is a mitogenic factor for microvascular endothelial cells: Its potential role in angiogenesis

Dean G. Tang, Colette Renaud, Sanja Stojakovic, Clement A. Diglio, Arther Porter, Kenneth V. Honn

Research output: Contribution to journal › Article › peer-review

95 Scopus citations

Abstract

12(S)-HETE [12(S)-hydroxyeicosatetraenoic acid] is a lipoxygenase metabolite of arachidonic acid. Treatment of murine-lung-derived microvascular endothelial cells (CD clone 4) with exogenous 12(S)-HETE promoted wound healing of injured endothelial cell monolayers. 12(S)-HETE, in a time- and dose-dependent manner, enhanced the growth of CD clone 4 cells. Thymidine incorporation assays demonstrated that 12(S)-HETE increased the DNA synthesis by > 4 fold. In addition, normal endothelial cell growth stimulated by serum could be dose-dependently inhibited by a select 12-lipoxygenase inhibitor (BHPP), suggesting that 12(S)-HETE is a physiological mitogenic factor for microvascular endothelial cells.

Original language	English (US)
Pages (from-to)	462-468
Number of pages	7
Journal	Biochemical and biophysical research communications
Volume	211
Issue number	2
DOIs	https://doi.org/10.1006/bbrc.1995.1836
State	Published - Jun 15 1995
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "12(S)-HETE is a mitogenic factor for microvascular endothelial cells: Its potential role in angiogenesis",

abstract = "12(S)-HETE [12(S)-hydroxyeicosatetraenoic acid] is a lipoxygenase metabolite of arachidonic acid. Treatment of murine-lung-derived microvascular endothelial cells (CD clone 4) with exogenous 12(S)-HETE promoted wound healing of injured endothelial cell monolayers. 12(S)-HETE, in a time- and dose-dependent manner, enhanced the growth of CD clone 4 cells. Thymidine incorporation assays demonstrated that 12(S)-HETE increased the DNA synthesis by > 4 fold. In addition, normal endothelial cell growth stimulated by serum could be dose-dependently inhibited by a select 12-lipoxygenase inhibitor (BHPP), suggesting that 12(S)-HETE is a physiological mitogenic factor for microvascular endothelial cells.",

author = "Tang, {Dean G.} and Colette Renaud and Sanja Stojakovic and Diglio, {Clement A.} and Arther Porter and Honn, {Kenneth V.}",

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T1 - 12(S)-HETE is a mitogenic factor for microvascular endothelial cells

T2 - Its potential role in angiogenesis

AU - Tang, Dean G.

AU - Renaud, Colette

AU - Stojakovic, Sanja

AU - Diglio, Clement A.

AU - Porter, Arther

AU - Honn, Kenneth V.

PY - 1995/6/15

Y1 - 1995/6/15

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AB - 12(S)-HETE [12(S)-hydroxyeicosatetraenoic acid] is a lipoxygenase metabolite of arachidonic acid. Treatment of murine-lung-derived microvascular endothelial cells (CD clone 4) with exogenous 12(S)-HETE promoted wound healing of injured endothelial cell monolayers. 12(S)-HETE, in a time- and dose-dependent manner, enhanced the growth of CD clone 4 cells. Thymidine incorporation assays demonstrated that 12(S)-HETE increased the DNA synthesis by > 4 fold. In addition, normal endothelial cell growth stimulated by serum could be dose-dependently inhibited by a select 12-lipoxygenase inhibitor (BHPP), suggesting that 12(S)-HETE is a physiological mitogenic factor for microvascular endothelial cells.

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12(S)-HETE is a mitogenic factor for microvascular endothelial cells: Its potential role in angiogenesis

Abstract

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