TY - JOUR
T1 - 2-Fluoro-ATP
T2 - A toxic metabolite of 9-β-D-arabinosyl-2-fluoroadenine
AU - Avramis, Vassilios I.
AU - Plunkett, William
PY - 1983/5/31
Y1 - 1983/5/31
N2 - Murine P388 cells incubated in vitro with the anticancer drug arabinosyl 2-fluoroadenine accumulate its 5′-triphosphate. F-araATP, as the major phosphorylated metabolite. A new chromatographically separate metabolite that accumulated to levels 10% of that of F-araATP was identified as 2-fluoro-ATP, by the following criteria. 1. The metabolite coeluted with the authentic compound on anio-exchange HPLC. 2. Dephosphorylation of the metabolite yielded a compound that was chromatographically identical to 2-fluoroadenosine. 3. The compound was sensitive to NaIO4 oxidation. Cellular incubation experiments indicated that 2-fluoroadenine, but not arabinosyl 2-fluorohydroxanthine, was the likely intermediate in the formation of 2-fluoro-ATP.
AB - Murine P388 cells incubated in vitro with the anticancer drug arabinosyl 2-fluoroadenine accumulate its 5′-triphosphate. F-araATP, as the major phosphorylated metabolite. A new chromatographically separate metabolite that accumulated to levels 10% of that of F-araATP was identified as 2-fluoro-ATP, by the following criteria. 1. The metabolite coeluted with the authentic compound on anio-exchange HPLC. 2. Dephosphorylation of the metabolite yielded a compound that was chromatographically identical to 2-fluoroadenosine. 3. The compound was sensitive to NaIO4 oxidation. Cellular incubation experiments indicated that 2-fluoroadenine, but not arabinosyl 2-fluorohydroxanthine, was the likely intermediate in the formation of 2-fluoro-ATP.
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U2 - 10.1016/0006-291X(83)90428-X
DO - 10.1016/0006-291X(83)90428-X
M3 - Article
C2 - 6860342
AN - SCOPUS:0020636463
SN - 0006-291X
VL - 113
SP - 35
EP - 43
JO - Biochemical and biophysical research communications
JF - Biochemical and biophysical research communications
IS - 1
ER -