2,5-Di(tert-butyl)-1,4-benzohydroquinone - a novel inhibitor of liver microsomal Ca2+ sequestration

Gregory A. Moore, David J. McConkey, Georges E.N. Kass, Peter J. O'Brien, Sten Orrenius

Research output: Contribution to journalArticle

159 Citations (Scopus)

Abstract

Treatment of rat liver microsomes with 2,5-di(tert-butyl)-1,4-benzohydroquinone caused a dose-related inhibition (Ki{reversed tilde equals}1 μM) of ATP-dependent Ca2+ sequestration. This was paralleled by a similar impairment of the microsomal Ca2+ -stimulated ATPase activity. In contrast, the hydroquinone failed to induce Ca2+ release from Ca2+ -loaded liver mitochondria (supplied with ATP), and inhibited neither the mitochondrial F1F0-ATPase nor the Ca2+ -stimulated ATPase activity of the hepatic plasma membrane fraction. The inhibition of microsomal Ca2+ sequestration was not associated with any apparent alteration of membrane permeability or loss of other microsomal enzyme activities or modification of microsomal protein thiols. These findings suggest that 2,5-di(tert-butyl)-1,4-benzohydroquinone is a potent and selective inhibitor of liver microsomal Ca2+ sequestration which may be a useful tool in studies of Ca2+ fluxes in intact cells and tissues.

Original languageEnglish (US)
Pages (from-to)331-336
Number of pages6
JournalFEBS Letters
Volume224
Issue number2
DOIs
StatePublished - Nov 30 1987

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Calcium-Transporting ATPases
Liver
Adenosine Triphosphatases
Adenosine Triphosphate
Liver Mitochondrion
Liver Microsomes
Sulfhydryl Compounds
Permeability
Mitochondria
Cell Membrane
Enzyme activity
Cell membranes
Membranes
Rats
Enzymes
Tissue
Fluxes
Proteins
2,5-di-tert-butylhydroquinone
hydroquinone

Keywords

  • (Ca + Mg)-ATPase
  • (Rat liver)
  • 2,5-Di(tert-butyl)-1,4-benzohydroquinone
  • Ca sequestration
  • Microsome

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Moore, G. A., McConkey, D. J., Kass, G. E. N., O'Brien, P. J., & Orrenius, S. (1987). 2,5-Di(tert-butyl)-1,4-benzohydroquinone - a novel inhibitor of liver microsomal Ca2+ sequestration. FEBS Letters, 224(2), 331-336. https://doi.org/10.1016/0014-5793(87)80479-9

2,5-Di(tert-butyl)-1,4-benzohydroquinone - a novel inhibitor of liver microsomal Ca2+ sequestration. / Moore, Gregory A.; McConkey, David J.; Kass, Georges E.N.; O'Brien, Peter J.; Orrenius, Sten.

In: FEBS Letters, Vol. 224, No. 2, 30.11.1987, p. 331-336.

Research output: Contribution to journalArticle

Moore, GA, McConkey, DJ, Kass, GEN, O'Brien, PJ & Orrenius, S 1987, '2,5-Di(tert-butyl)-1,4-benzohydroquinone - a novel inhibitor of liver microsomal Ca2+ sequestration', FEBS Letters, vol. 224, no. 2, pp. 331-336. https://doi.org/10.1016/0014-5793(87)80479-9
Moore, Gregory A. ; McConkey, David J. ; Kass, Georges E.N. ; O'Brien, Peter J. ; Orrenius, Sten. / 2,5-Di(tert-butyl)-1,4-benzohydroquinone - a novel inhibitor of liver microsomal Ca2+ sequestration. In: FEBS Letters. 1987 ; Vol. 224, No. 2. pp. 331-336.
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