(6–4)Photoproducts are removed from the DNA of UV-irradiated mammalian cells more efficiently than cyclobutane pyrimidine dimers

D. L. Mitchell; C. A. Haipek; J. M. Clarkson

doi:10.1016/S0165-7992(85)80018-X

(6–4)Photoproducts are removed from the DNA of UV-irradiated mammalian cells more efficiently than cyclobutane pyrimidine dimers

D. L. Mitchell, C. A. Haipek, J. M. Clarkson

Epigenetics & Molecular Carcinogenesis

Research output: Contribution to journal › Article › peer-review

251 Scopus citations

Abstract

A polyclonal antiserum raised against UV-irradiated DNA can be used to assay cyclobutane pyrimidine dimers and Pyr(6-4)Pyo photoproducts specifically by changing the nature of the ³²P-labelled antigen. Pyr(6-4)Pyo photoproducts were removed faster than cyclobutane dimers in UV-irradiated human, hamster and mouse cells. Xeroderma pigmentosum cells from complementation groups A, C and D were deficient in the repair of both lesions.

Original language	English (US)
Pages (from-to)	109-112
Number of pages	4
Journal	Mutation Research - Mutation Research Letters
Volume	143
Issue number	3
DOIs	https://doi.org/10.1016/S0165-7992(85)80018-X
State	Published - 1985

ASJC Scopus subject areas

General Medicine

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10.1016/S0165-7992(85)80018-X

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title = "(6–4)Photoproducts are removed from the DNA of UV-irradiated mammalian cells more efficiently than cyclobutane pyrimidine dimers",

abstract = "A polyclonal antiserum raised against UV-irradiated DNA can be used to assay cyclobutane pyrimidine dimers and Pyr(6-4)Pyo photoproducts specifically by changing the nature of the 32P-labelled antigen. Pyr(6-4)Pyo photoproducts were removed faster than cyclobutane dimers in UV-irradiated human, hamster and mouse cells. Xeroderma pigmentosum cells from complementation groups A, C and D were deficient in the repair of both lesions.",

author = "Mitchell, {D. L.} and Haipek, {C. A.} and Clarkson, {J. M.}",

year = "1985",

doi = "10.1016/S0165-7992(85)80018-X",

language = "English (US)",

volume = "143",

pages = "109--112",

journal = "Mutation Research - Mutation Research Letters",

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AU - Mitchell, D. L.

AU - Haipek, C. A.

AU - Clarkson, J. M.

PY - 1985

Y1 - 1985

N2 - A polyclonal antiserum raised against UV-irradiated DNA can be used to assay cyclobutane pyrimidine dimers and Pyr(6-4)Pyo photoproducts specifically by changing the nature of the 32P-labelled antigen. Pyr(6-4)Pyo photoproducts were removed faster than cyclobutane dimers in UV-irradiated human, hamster and mouse cells. Xeroderma pigmentosum cells from complementation groups A, C and D were deficient in the repair of both lesions.

AB - A polyclonal antiserum raised against UV-irradiated DNA can be used to assay cyclobutane pyrimidine dimers and Pyr(6-4)Pyo photoproducts specifically by changing the nature of the 32P-labelled antigen. Pyr(6-4)Pyo photoproducts were removed faster than cyclobutane dimers in UV-irradiated human, hamster and mouse cells. Xeroderma pigmentosum cells from complementation groups A, C and D were deficient in the repair of both lesions.

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ER -

(6–4)Photoproducts are removed from the DNA of UV-irradiated mammalian cells more efficiently than cyclobutane pyrimidine dimers

Abstract

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