A 10-color flow cytometry panel for diagnosis and minimal residual disease in chronic lymphocytic leukemia

Alexandre Bazinet; Ryan N. Rys; Amadou Barry; Celia M.T. Greenwood; Yoon Kow Young; Alma Mendoza; Ida LaPorta; Claudia M. Wever; François E. Mercier; Nathalie A. Johnson

doi:10.1080/10428194.2021.1919658

A 10-color flow cytometry panel for diagnosis and minimal residual disease in chronic lymphocytic leukemia

Alexandre Bazinet, Ryan N. Rys, Amadou Barry, Celia M.T. Greenwood, Yoon Kow Young, Alma Mendoza, Ida LaPorta, Claudia M. Wever, François E. Mercier, Nathalie A. Johnson

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Diagnosis and minimal residual disease (MRD) monitoring of chronic lymphocytic leukemia (CLL) by flow cytometry currently requires multiple antibody panels. We added CD23 and CD200 to the EuroFlow^TM lymphoid screening tube (LST) to create a 10-color modified LST (mLST) capable of diagnosing typical CLL in a single tube. We then explored if the mLST could be used for MRD by comparing its performance to the European Research Initiative on CLL (ERIC) panel using spiked cryopreserved and fresh patient samples. Over 1 year of use in our clinical laboratory, the mLST diagnosed CLL without further immunophenotyping in 56% of samples with an abnormal clone. There was good agreement in MRD results between the mLST and ERIC panels. Therefore, the mLST can streamline CLL diagnosis by reducing technician time and the number of panels required. It may have the potential to screen for MRD in laboratories without access to dedicated panels (ERIC).

Original language	English (US)
Pages (from-to)	2352-2359
Number of pages	8
Journal	Leukemia and Lymphoma
Volume	62
Issue number	10
DOIs	https://doi.org/10.1080/10428194.2021.1919658
State	Published - 2021
Externally published	Yes

Keywords

Chronic lymphocytic leukemia
diagnostic testing
flow cytometry
minimal residual disease

ASJC Scopus subject areas

Hematology
Oncology
Cancer Research

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10.1080/10428194.2021.1919658

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title = "A 10-color flow cytometry panel for diagnosis and minimal residual disease in chronic lymphocytic leukemia",

abstract = "Diagnosis and minimal residual disease (MRD) monitoring of chronic lymphocytic leukemia (CLL) by flow cytometry currently requires multiple antibody panels. We added CD23 and CD200 to the EuroFlowTM lymphoid screening tube (LST) to create a 10-color modified LST (mLST) capable of diagnosing typical CLL in a single tube. We then explored if the mLST could be used for MRD by comparing its performance to the European Research Initiative on CLL (ERIC) panel using spiked cryopreserved and fresh patient samples. Over 1 year of use in our clinical laboratory, the mLST diagnosed CLL without further immunophenotyping in 56% of samples with an abnormal clone. There was good agreement in MRD results between the mLST and ERIC panels. Therefore, the mLST can streamline CLL diagnosis by reducing technician time and the number of panels required. It may have the potential to screen for MRD in laboratories without access to dedicated panels (ERIC).",

keywords = "Chronic lymphocytic leukemia, diagnostic testing, flow cytometry, minimal residual disease",

author = "Alexandre Bazinet and Rys, {Ryan N.} and Amadou Barry and Greenwood, {Celia M.T.} and Young, {Yoon Kow} and Alma Mendoza and Ida LaPorta and Wever, {Claudia M.} and Mercier, {Fran{\c c}ois E.} and Johnson, {Nathalie A.}",

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year = "2021",

doi = "10.1080/10428194.2021.1919658",

language = "English (US)",

volume = "62",

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T1 - A 10-color flow cytometry panel for diagnosis and minimal residual disease in chronic lymphocytic leukemia

AU - Bazinet, Alexandre

AU - Rys, Ryan N.

AU - Barry, Amadou

AU - Greenwood, Celia M.T.

AU - Young, Yoon Kow

AU - Mendoza, Alma

AU - LaPorta, Ida

AU - Wever, Claudia M.

AU - Mercier, François E.

AU - Johnson, Nathalie A.

PY - 2021

Y1 - 2021

N2 - Diagnosis and minimal residual disease (MRD) monitoring of chronic lymphocytic leukemia (CLL) by flow cytometry currently requires multiple antibody panels. We added CD23 and CD200 to the EuroFlowTM lymphoid screening tube (LST) to create a 10-color modified LST (mLST) capable of diagnosing typical CLL in a single tube. We then explored if the mLST could be used for MRD by comparing its performance to the European Research Initiative on CLL (ERIC) panel using spiked cryopreserved and fresh patient samples. Over 1 year of use in our clinical laboratory, the mLST diagnosed CLL without further immunophenotyping in 56% of samples with an abnormal clone. There was good agreement in MRD results between the mLST and ERIC panels. Therefore, the mLST can streamline CLL diagnosis by reducing technician time and the number of panels required. It may have the potential to screen for MRD in laboratories without access to dedicated panels (ERIC).

AB - Diagnosis and minimal residual disease (MRD) monitoring of chronic lymphocytic leukemia (CLL) by flow cytometry currently requires multiple antibody panels. We added CD23 and CD200 to the EuroFlowTM lymphoid screening tube (LST) to create a 10-color modified LST (mLST) capable of diagnosing typical CLL in a single tube. We then explored if the mLST could be used for MRD by comparing its performance to the European Research Initiative on CLL (ERIC) panel using spiked cryopreserved and fresh patient samples. Over 1 year of use in our clinical laboratory, the mLST diagnosed CLL without further immunophenotyping in 56% of samples with an abnormal clone. There was good agreement in MRD results between the mLST and ERIC panels. Therefore, the mLST can streamline CLL diagnosis by reducing technician time and the number of panels required. It may have the potential to screen for MRD in laboratories without access to dedicated panels (ERIC).

KW - Chronic lymphocytic leukemia

KW - diagnostic testing

KW - flow cytometry

KW - minimal residual disease

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A 10-color flow cytometry panel for diagnosis and minimal residual disease in chronic lymphocytic leukemia

Abstract

Keywords

ASJC Scopus subject areas

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