A colorimetric liquid culture assay of a growth factor for primitive murine macrophage progenitor cells

A. B. Kriegler; T. R. Bradley; G. S. Hodgson; I. K. McNiece

doi:10.1016/0022-1759(87)90246-8

A colorimetric liquid culture assay of a growth factor for primitive murine macrophage progenitor cells

A. B. Kriegler, T. R. Bradley, G. S. Hodgson, I. K. McNiece

Stem Cell Transplantation

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Synergistic factors from media conditioned (CM) by human placentas or the 5637 human bladder carcinoma cell line (SF_H-HPCM and SF_H-5637 respectively) have the ability to stimulate early progenitor cells in mouse bone marrow to form large colonies in agar cultures after 12-14 days, in the presence of CSF-1. Culture conditions have been examined and a quicker and more convenient liquid culture assay has been developed for this factor, using a tetrazolium salt to quantitate cell proliferation. The use of flat-bottomed vessels, high cell density, supra-optimal doses of CSF-1 or the addition of WEHI-3-CM to these cultures, all resulted in a decrease in the required incubation time. In combination, these modifications reduced the assay time to 4 days.

Original language	English (US)
Pages (from-to)	93-102
Number of pages	10
Journal	Journal of Immunological Methods
Volume	103
Issue number	1
DOIs	https://doi.org/10.1016/0022-1759(87)90246-8
State	Published - Oct 23 1987

Keywords

Hemopoietin 1
High proliferative potential colony-forming cell
Liquid culture
Synergistic factor

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/0022-1759(87)90246-8

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title = "A colorimetric liquid culture assay of a growth factor for primitive murine macrophage progenitor cells",

abstract = "Synergistic factors from media conditioned (CM) by human placentas or the 5637 human bladder carcinoma cell line (SFH-HPCM and SFH-5637 respectively) have the ability to stimulate early progenitor cells in mouse bone marrow to form large colonies in agar cultures after 12-14 days, in the presence of CSF-1. Culture conditions have been examined and a quicker and more convenient liquid culture assay has been developed for this factor, using a tetrazolium salt to quantitate cell proliferation. The use of flat-bottomed vessels, high cell density, supra-optimal doses of CSF-1 or the addition of WEHI-3-CM to these cultures, all resulted in a decrease in the required incubation time. In combination, these modifications reduced the assay time to 4 days.",

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T1 - A colorimetric liquid culture assay of a growth factor for primitive murine macrophage progenitor cells

AU - Kriegler, A. B.

AU - Bradley, T. R.

AU - Hodgson, G. S.

AU - McNiece, I. K.

PY - 1987/10/23

Y1 - 1987/10/23

N2 - Synergistic factors from media conditioned (CM) by human placentas or the 5637 human bladder carcinoma cell line (SFH-HPCM and SFH-5637 respectively) have the ability to stimulate early progenitor cells in mouse bone marrow to form large colonies in agar cultures after 12-14 days, in the presence of CSF-1. Culture conditions have been examined and a quicker and more convenient liquid culture assay has been developed for this factor, using a tetrazolium salt to quantitate cell proliferation. The use of flat-bottomed vessels, high cell density, supra-optimal doses of CSF-1 or the addition of WEHI-3-CM to these cultures, all resulted in a decrease in the required incubation time. In combination, these modifications reduced the assay time to 4 days.

AB - Synergistic factors from media conditioned (CM) by human placentas or the 5637 human bladder carcinoma cell line (SFH-HPCM and SFH-5637 respectively) have the ability to stimulate early progenitor cells in mouse bone marrow to form large colonies in agar cultures after 12-14 days, in the presence of CSF-1. Culture conditions have been examined and a quicker and more convenient liquid culture assay has been developed for this factor, using a tetrazolium salt to quantitate cell proliferation. The use of flat-bottomed vessels, high cell density, supra-optimal doses of CSF-1 or the addition of WEHI-3-CM to these cultures, all resulted in a decrease in the required incubation time. In combination, these modifications reduced the assay time to 4 days.

KW - Hemopoietin 1

KW - High proliferative potential colony-forming cell

KW - Liquid culture

KW - Synergistic factor

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A colorimetric liquid culture assay of a growth factor for primitive murine macrophage progenitor cells

Abstract

Keywords

ASJC Scopus subject areas

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