TY - JOUR
T1 - A Direct analysis of methotrexate, dichloromethotrexate and their 7-hydroxy metabolites in plasma by high pressure liquid chromatography
AU - Tong, William P.
AU - Wisnicki, Jeffrey L.
AU - Horton, John
AU - Ludlum, David B.
N1 - Funding Information:
This work is supported by Public Health Service Grant CA 20129 from the National Cancer Institute (NCI), National Institutes of Health, Department of Health, Education, and Welfare.
PY - 1980
Y1 - 1980
N2 - Plasma levels of methotrexate, dichloromethotrexate, and their 7-hydroxy metabolites can be determined quickly and accurately by direct analysis in a high pressure liquid chromatographic system. This system utilizes a precolumn of CO:Pell ODS resin to protect the analytical μ-Bondapak C18 column. The precolumn also provides a bed for extraction of drugs from the plasma proteins which are trapped in it. Elution with a paired ion solvent provides good recovery of methotrexate from the plasma and excellent separation on the column. The primary advantages of this system are its speed and simplicity combined with its ability to determine the plasma level of parent drug and its metabolite simultaneously.
AB - Plasma levels of methotrexate, dichloromethotrexate, and their 7-hydroxy metabolites can be determined quickly and accurately by direct analysis in a high pressure liquid chromatographic system. This system utilizes a precolumn of CO:Pell ODS resin to protect the analytical μ-Bondapak C18 column. The precolumn also provides a bed for extraction of drugs from the plasma proteins which are trapped in it. Elution with a paired ion solvent provides good recovery of methotrexate from the plasma and excellent separation on the column. The primary advantages of this system are its speed and simplicity combined with its ability to determine the plasma level of parent drug and its metabolite simultaneously.
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U2 - 10.1016/0009-8981(80)90415-5
DO - 10.1016/0009-8981(80)90415-5
M3 - Article
C2 - 7428179
AN - SCOPUS:0018956797
SN - 0009-8981
VL - 107
SP - 67
EP - 72
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
IS - 1-2
ER -