TY - JOUR
T1 - A direct cytogenetic technique for mouse skin carcinomas and papillomas
AU - Aldaz, Claudio M.
AU - Conti, Claudio J.
AU - Klein-Szanto, Andres J.P.
AU - Slaga, Thomas J.
N1 - Funding Information:
This work was supported by Grants CA 34890, CA 34962, and CA 34521. We wish to express our appreciation to JoAnn Fries for excellent technical assistance; to Joyce Mayhugh for typing this manuscript; to Barbara Reschke for editorial suggestions; and to John Riley and Judy Ing for art and photographic assistance.
PY - 1986/2/15
Y1 - 1986/2/15
N2 - A method was developed for obtaining direct chromosome preparations from SENCAR mouse skin tumors induced by chemical carcinogenesis protocols. Papillomas and squamous cell carcinomas were mechanically dispersed immediately after resection and were placed in a modified Hanks' solution with collagenase, trypsin, hyaluronidase, bovine albumin, and Colcemid. Total exposure to Colcemid did not exceed 1 hr. Metaphases were obtained in 100% of the analyzed specimens, allowing chromosome counting screening for double minutes and, in 50% of the cases, useful G-banded slides. The technique described has produced, for this type of tumor, a higher number of successful G-banded preparations than other previously reported methods for solid tumors. This procedure may be applicable for the study of human solid tumors that are histologically similar to our murine model, such as squamous cell carcinoma of cervix or lung.
AB - A method was developed for obtaining direct chromosome preparations from SENCAR mouse skin tumors induced by chemical carcinogenesis protocols. Papillomas and squamous cell carcinomas were mechanically dispersed immediately after resection and were placed in a modified Hanks' solution with collagenase, trypsin, hyaluronidase, bovine albumin, and Colcemid. Total exposure to Colcemid did not exceed 1 hr. Metaphases were obtained in 100% of the analyzed specimens, allowing chromosome counting screening for double minutes and, in 50% of the cases, useful G-banded slides. The technique described has produced, for this type of tumor, a higher number of successful G-banded preparations than other previously reported methods for solid tumors. This procedure may be applicable for the study of human solid tumors that are histologically similar to our murine model, such as squamous cell carcinoma of cervix or lung.
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U2 - 10.1016/0165-4608(86)90077-4
DO - 10.1016/0165-4608(86)90077-4
M3 - Article
C2 - 3943063
AN - SCOPUS:0022611079
SN - 0165-4608
VL - 20
SP - 223
EP - 229
JO - Cancer Genetics and Cytogenetics
JF - Cancer Genetics and Cytogenetics
IS - 3-4
ER -