A disposable diffusion chamber system used to study immunoregulation by soluble factors

A disposable two-chamber culture system is described which is useful for studying various soluble factors which regulate the immune response. This method requires small numbers of cells and less than 2 ml of medium per culture. The vessel is compact, relatively inexpensive and all component parts are readily available from commercial suppliers inexpensive and all component parts are readily available from commercial suppliers. This culture system circumvents the major problems associated with dual chamber methods previously developed for the study of immunoregulatory molecules affecting antibody production.