Abstract
A general method was developed for the isolation of Salmonella thyphimurium LT2 Mu d1-8 (Aprlac) operon fusions in a gene displacing a Tn 10 insertion. Random Mu d1-8 fusion pools were prepared to grow phage P22 lysates which transduced chlC::Tn 10 to AprTets on fusaric acidampicillin plates. Among these AprTets potential chlC::Mu d1-8 fusions, a simple spot test identified the fusions that were closely linked to the Tn 10 insertion in chlC. Out of 68 AprTets isolates 7 chlC::Mu d1-8 fusions with a nitrate-induced Lac+ phenotype were obtained. When oxrA::Tn 10 was transduced into these chlC::Mu d1-8 fusions, they became Lac- even in the presence of nitrate, confirming that they were chlC::Mu d1-8 fusions.
Original language | English (US) |
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Pages (from-to) | 221-224 |
Number of pages | 4 |
Journal | MGG Molecular & General Genetics |
Volume | 205 |
Issue number | 2 |
DOIs | |
State | Published - Nov 1986 |
Keywords
- Fusion pools
- Nitrate reductase
- oxrA
ASJC Scopus subject areas
- Genetics