A homozygous p53 R282W mutant human embryonic stem cell line generated using TALEN-mediated precise gene editing

Ruoji Zhou; An Xu; Donghui Wang; Dandan Zhu; Helen Mata; Zijun Huo; Jian Tu; Mo Liu; Alaa M.T. Mohamed; Brittany E. Jewell; Julian Gingold; Weiya Xia; Pulivarthi H. Rao; Mien Chie Hung; Ruiying Zhao; Dung Fang Lee

doi:10.1016/j.scr.2018.01.035

A homozygous p53 R282W mutant human embryonic stem cell line generated using TALEN-mediated precise gene editing

Ruoji Zhou, An Xu, Donghui Wang, Dandan Zhu, Helen Mata, Zijun Huo, Jian Tu, Mo Liu, Alaa M.T. Mohamed, Brittany E. Jewell, Julian Gingold, Weiya Xia, Pulivarthi H. Rao, Mien Chie Hung, Ruiying Zhao, Dung Fang Lee

Molecular & Cellular Oncology

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

The tumor suppressor gene TP53 is the most frequently mutated gene in human cancers. Many hot-spot mutations of TP53 confer novel functions not found in wild-type p53 and contribute to tumor development and progression. We report on the generation of a H1 human embryonic stem cell line carrying a homozygous TP53 R282W mutation using TALEN-mediated genome editing. The generated cell line demonstrates normal karyotype, maintains a pluripotent state, and is capable of generating a teratoma in vivo containing tissues from all three germ layers.

Original language	English (US)
Pages (from-to)	131-135
Number of pages	5
Journal	Stem Cell Research
Volume	27
DOIs	https://doi.org/10.1016/j.scr.2018.01.035
State	Published - Mar 2018

ASJC Scopus subject areas

Developmental Biology
Cell Biology

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Zhou, R., Xu, A., Wang, D., Zhu, D., Mata, H., Huo, Z., Tu, J., Liu, M., Mohamed, A. M. T., Jewell, B. E., Gingold, J., Xia, W., Rao, P. H., Hung, M. C., Zhao, R., & Lee, D. F. (2018). A homozygous p53 R282W mutant human embryonic stem cell line generated using TALEN-mediated precise gene editing. Stem Cell Research, 27, 131-135. https://doi.org/10.1016/j.scr.2018.01.035

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title = "A homozygous p53 R282W mutant human embryonic stem cell line generated using TALEN-mediated precise gene editing",

abstract = "The tumor suppressor gene TP53 is the most frequently mutated gene in human cancers. Many hot-spot mutations of TP53 confer novel functions not found in wild-type p53 and contribute to tumor development and progression. We report on the generation of a H1 human embryonic stem cell line carrying a homozygous TP53 R282W mutation using TALEN-mediated genome editing. The generated cell line demonstrates normal karyotype, maintains a pluripotent state, and is capable of generating a teratoma in vivo containing tissues from all three germ layers.",

author = "Ruoji Zhou and An Xu and Donghui Wang and Dandan Zhu and Helen Mata and Zijun Huo and Jian Tu and Mo Liu and Mohamed, {Alaa M.T.} and Jewell, {Brittany E.} and Julian Gingold and Weiya Xia and Rao, {Pulivarthi H.} and Hung, {Mien Chie} and Ruiying Zhao and Lee, {Dung Fang}",

note = "Publisher Copyright: {\textcopyright} 2018 The Author(s)",

year = "2018",

month = mar,

doi = "10.1016/j.scr.2018.01.035",

language = "English (US)",

volume = "27",

pages = "131--135",

journal = "Stem Cell Research",

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T1 - A homozygous p53 R282W mutant human embryonic stem cell line generated using TALEN-mediated precise gene editing

AU - Zhou, Ruoji

AU - Xu, An

AU - Wang, Donghui

AU - Zhu, Dandan

AU - Mata, Helen

AU - Huo, Zijun

AU - Tu, Jian

AU - Liu, Mo

AU - Mohamed, Alaa M.T.

AU - Jewell, Brittany E.

AU - Gingold, Julian

AU - Xia, Weiya

AU - Rao, Pulivarthi H.

AU - Hung, Mien Chie

AU - Zhao, Ruiying

AU - Lee, Dung Fang

PY - 2018/3

Y1 - 2018/3

N2 - The tumor suppressor gene TP53 is the most frequently mutated gene in human cancers. Many hot-spot mutations of TP53 confer novel functions not found in wild-type p53 and contribute to tumor development and progression. We report on the generation of a H1 human embryonic stem cell line carrying a homozygous TP53 R282W mutation using TALEN-mediated genome editing. The generated cell line demonstrates normal karyotype, maintains a pluripotent state, and is capable of generating a teratoma in vivo containing tissues from all three germ layers.

AB - The tumor suppressor gene TP53 is the most frequently mutated gene in human cancers. Many hot-spot mutations of TP53 confer novel functions not found in wild-type p53 and contribute to tumor development and progression. We report on the generation of a H1 human embryonic stem cell line carrying a homozygous TP53 R282W mutation using TALEN-mediated genome editing. The generated cell line demonstrates normal karyotype, maintains a pluripotent state, and is capable of generating a teratoma in vivo containing tissues from all three germ layers.

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EP - 135

JO - Stem Cell Research

JF - Stem Cell Research

ER -

A homozygous p53 R282W mutant human embryonic stem cell line generated using TALEN-mediated precise gene editing

Abstract

ASJC Scopus subject areas

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