A microRNA expression signature of human solid tumors defines cancer gene targets

Stefano Volinia; George A. Calin; Chang Gong Liu; Stefan Ambs; Amelia Cimmino; Fabio Petrocca; Rosa Visone; Marilena Iorio; Claudia Roldo; Manuela Ferracin; Robyn L. Prueitt; Nozumu Yanaihara; Giovanni Lanza; Aldo Scarpa; Andrea Vecchione; Massimo Negrini; Curtis C. Harris; Carlo M. Croce

doi:10.1073/pnas.0510565103

A microRNA expression signature of human solid tumors defines cancer gene targets

Stefano Volinia, George A. Calin, Chang Gong Liu, Stefan Ambs, Amelia Cimmino, Fabio Petrocca, Rosa Visone, Marilena Iorio, Claudia Roldo, Manuela Ferracin, Robyn L. Prueitt, Nozumu Yanaihara, Giovanni Lanza, Aldo Scarpa, Andrea Vecchione, Massimo Negrini, Curtis C. Harris, Carlo M. Croce

Research output: Contribution to journal › Article › peer-review

5108 Scopus citations

Abstract

Small noncoding microRNAs (miRNAs) can contribute to cancer development and progression and are differentially expressed in normal tissues and cancers. From a large-scale miRnome analysis on 540 samples including lung, breast, stomach, prostate, colon, and pancreatic tumors, we identified a solid cancer miRNA signature composed by a large portion of overexpressed miRNAs. Among these miRNAs are some with well characterized cancer association, such as miR-17-5p, miR-20a, miR-21, miR-92, miR-106a, and miR-155. The predicted targets for the differentially expressed miRNAs are significantly enriched for protein-coding tumor suppressors and oncogenes (P < 0.0001). A number of the predicted targets, including the tumor suppressors RB1 (Retinoblastoma 1) and TGFBR2 (transforming growth factor, beta receptor II) genes were confirmed experimentally. Our results indicate that miRNAs are extensively involved in cancer pathogenesis of solid tumors and support their function as either dominant or recessive cancer genes.

Original language	English (US)
Pages (from-to)	2257-2261
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	103
Issue number	7
DOIs	https://doi.org/10.1073/pnas.0510565103
State	Published - Feb 14 2006
Externally published	Yes

Keywords

Microarray
Transcriptome
Tumorigenesis

ASJC Scopus subject areas

General

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10.1073/pnas.0510565103

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Volinia, S., Calin, G. A., Liu, C. G., Ambs, S., Cimmino, A., Petrocca, F., Visone, R., Iorio, M., Roldo, C., Ferracin, M., Prueitt, R. L., Yanaihara, N., Lanza, G., Scarpa, A., Vecchione, A., Negrini, M., Harris, C. C., & Croce, C. M. (2006). A microRNA expression signature of human solid tumors defines cancer gene targets. Proceedings of the National Academy of Sciences of the United States of America, 103(7), 2257-2261. https://doi.org/10.1073/pnas.0510565103

Volinia, S, Calin, GA, Liu, CG, Ambs, S, Cimmino, A, Petrocca, F, Visone, R, Iorio, M, Roldo, C, Ferracin, M, Prueitt, RL, Yanaihara, N, Lanza, G, Scarpa, A, Vecchione, A, Negrini, M, Harris, CC & Croce, CM 2006, 'A microRNA expression signature of human solid tumors defines cancer gene targets', Proceedings of the National Academy of Sciences of the United States of America, vol. 103, no. 7, pp. 2257-2261. https://doi.org/10.1073/pnas.0510565103

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abstract = "Small noncoding microRNAs (miRNAs) can contribute to cancer development and progression and are differentially expressed in normal tissues and cancers. From a large-scale miRnome analysis on 540 samples including lung, breast, stomach, prostate, colon, and pancreatic tumors, we identified a solid cancer miRNA signature composed by a large portion of overexpressed miRNAs. Among these miRNAs are some with well characterized cancer association, such as miR-17-5p, miR-20a, miR-21, miR-92, miR-106a, and miR-155. The predicted targets for the differentially expressed miRNAs are significantly enriched for protein-coding tumor suppressors and oncogenes (P < 0.0001). A number of the predicted targets, including the tumor suppressors RB1 (Retinoblastoma 1) and TGFBR2 (transforming growth factor, beta receptor II) genes were confirmed experimentally. Our results indicate that miRNAs are extensively involved in cancer pathogenesis of solid tumors and support their function as either dominant or recessive cancer genes.",

keywords = "Microarray, Transcriptome, Tumorigenesis",

author = "Stefano Volinia and Calin, {George A.} and Liu, {Chang Gong} and Stefan Ambs and Amelia Cimmino and Fabio Petrocca and Rosa Visone and Marilena Iorio and Claudia Roldo and Manuela Ferracin and Prueitt, {Robyn L.} and Nozumu Yanaihara and Giovanni Lanza and Aldo Scarpa and Andrea Vecchione and Massimo Negrini and Harris, {Curtis C.} and Croce, {Carlo M.}",

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AU - Volinia, Stefano

AU - Calin, George A.

AU - Liu, Chang Gong

AU - Ambs, Stefan

AU - Cimmino, Amelia

AU - Petrocca, Fabio

AU - Visone, Rosa

AU - Iorio, Marilena

AU - Roldo, Claudia

AU - Ferracin, Manuela

AU - Prueitt, Robyn L.

AU - Yanaihara, Nozumu

AU - Lanza, Giovanni

AU - Scarpa, Aldo

AU - Vecchione, Andrea

AU - Negrini, Massimo

AU - Harris, Curtis C.

AU - Croce, Carlo M.

PY - 2006/2/14

Y1 - 2006/2/14

N2 - Small noncoding microRNAs (miRNAs) can contribute to cancer development and progression and are differentially expressed in normal tissues and cancers. From a large-scale miRnome analysis on 540 samples including lung, breast, stomach, prostate, colon, and pancreatic tumors, we identified a solid cancer miRNA signature composed by a large portion of overexpressed miRNAs. Among these miRNAs are some with well characterized cancer association, such as miR-17-5p, miR-20a, miR-21, miR-92, miR-106a, and miR-155. The predicted targets for the differentially expressed miRNAs are significantly enriched for protein-coding tumor suppressors and oncogenes (P < 0.0001). A number of the predicted targets, including the tumor suppressors RB1 (Retinoblastoma 1) and TGFBR2 (transforming growth factor, beta receptor II) genes were confirmed experimentally. Our results indicate that miRNAs are extensively involved in cancer pathogenesis of solid tumors and support their function as either dominant or recessive cancer genes.

AB - Small noncoding microRNAs (miRNAs) can contribute to cancer development and progression and are differentially expressed in normal tissues and cancers. From a large-scale miRnome analysis on 540 samples including lung, breast, stomach, prostate, colon, and pancreatic tumors, we identified a solid cancer miRNA signature composed by a large portion of overexpressed miRNAs. Among these miRNAs are some with well characterized cancer association, such as miR-17-5p, miR-20a, miR-21, miR-92, miR-106a, and miR-155. The predicted targets for the differentially expressed miRNAs are significantly enriched for protein-coding tumor suppressors and oncogenes (P < 0.0001). A number of the predicted targets, including the tumor suppressors RB1 (Retinoblastoma 1) and TGFBR2 (transforming growth factor, beta receptor II) genes were confirmed experimentally. Our results indicate that miRNAs are extensively involved in cancer pathogenesis of solid tumors and support their function as either dominant or recessive cancer genes.

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A microRNA expression signature of human solid tumors defines cancer gene targets

Abstract

Keywords

ASJC Scopus subject areas

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