A modified fluorescence in situ hybridization (FISH) technique

Asha S. Multani; Vicki L. Hopwood; Sen Pathak

A modified fluorescence in situ hybridization (FISH) technique

Asha S. Multani, Vicki L. Hopwood, Sen Pathak

Research output: Contribution to journal › Article › peer-review

Abstract

We have modified and shortened the routine fluorescence in situ hybridization (FISH) technique by using microwave or formalin pretreatment for artificially aging and microwave heating only for dehydrating the regular chromosome preparations. The all-human telomere probe (Oncor, Inc., Gaithersburg, MD) was used on murine K-1735 clone X-21 melanoma cells for this purpose. The intensity of signais obtained by our modified technique(s) was comparable to that obtained by the routine procedure. However, pretreatment of slides with hydrogen peroxide (H₂O₂) completely obliterates the FISH signals.

Original language	English (US)
Pages (from-to)	3435-3438
Number of pages	4
Journal	Anticancer research
Volume	16
Issue number	6 B
State	Published - 1996

Keywords

DNA probe
Fluorescence in situ hybridization (FISH)
Premature chromosome condensation (FCC)
Telomeric signals, microwave

ASJC Scopus subject areas

Oncology
Cancer Research

Cite this

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title = "A modified fluorescence in situ hybridization (FISH) technique",

abstract = "We have modified and shortened the routine fluorescence in situ hybridization (FISH) technique by using microwave or formalin pretreatment for artificially aging and microwave heating only for dehydrating the regular chromosome preparations. The all-human telomere probe (Oncor, Inc., Gaithersburg, MD) was used on murine K-1735 clone X-21 melanoma cells for this purpose. The intensity of signais obtained by our modified technique(s) was comparable to that obtained by the routine procedure. However, pretreatment of slides with hydrogen peroxide (H2O2) completely obliterates the FISH signals.",

keywords = "DNA probe, Fluorescence in situ hybridization (FISH), Premature chromosome condensation (FCC), Telomeric signals, microwave",

author = "Multani, {Asha S.} and Hopwood, {Vicki L.} and Sen Pathak",

year = "1996",

language = "English (US)",

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pages = "3435--3438",

journal = "Anticancer research",

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T1 - A modified fluorescence in situ hybridization (FISH) technique

AU - Multani, Asha S.

AU - Hopwood, Vicki L.

AU - Pathak, Sen

PY - 1996

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N2 - We have modified and shortened the routine fluorescence in situ hybridization (FISH) technique by using microwave or formalin pretreatment for artificially aging and microwave heating only for dehydrating the regular chromosome preparations. The all-human telomere probe (Oncor, Inc., Gaithersburg, MD) was used on murine K-1735 clone X-21 melanoma cells for this purpose. The intensity of signais obtained by our modified technique(s) was comparable to that obtained by the routine procedure. However, pretreatment of slides with hydrogen peroxide (H2O2) completely obliterates the FISH signals.

AB - We have modified and shortened the routine fluorescence in situ hybridization (FISH) technique by using microwave or formalin pretreatment for artificially aging and microwave heating only for dehydrating the regular chromosome preparations. The all-human telomere probe (Oncor, Inc., Gaithersburg, MD) was used on murine K-1735 clone X-21 melanoma cells for this purpose. The intensity of signais obtained by our modified technique(s) was comparable to that obtained by the routine procedure. However, pretreatment of slides with hydrogen peroxide (H2O2) completely obliterates the FISH signals.

KW - DNA probe

KW - Fluorescence in situ hybridization (FISH)

KW - Premature chromosome condensation (FCC)

KW - Telomeric signals, microwave

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AN - SCOPUS:0030514285

SN - 0250-7005

VL - 16

SP - 3435

EP - 3438

JO - Anticancer research

JF - Anticancer research

IS - 6 B

ER -

A modified fluorescence in situ hybridization (FISH) technique

Abstract

Keywords

ASJC Scopus subject areas

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