A multimodal targeting nanoparticle for selectively labeling T. cells

Jonathan Gunn; Herschel Wallen; Omid Veiseh; Conroy Sun; Chen Fang; Jianhong Cao; Cassian Yee; Miqin Zhang

doi:10.1002/smll.200701103

A multimodal targeting nanoparticle for selectively labeling T. cells

Jonathan Gunn, Herschel Wallen, Omid Veiseh, Conroy Sun, Chen Fang, Jianhong Cao, Cassian Yee, Miqin Zhang

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

A study was conducted to demonstrate a dual-modality nanoparticle system, capable of labeling and imaging CTL cells, expressing T-cell receptors (TCR) that recognize cognate MHC-peptide complexes on the surface of antigen-presenting tumor cells. The study demonstrated in-vivo T-cell selectivity and reporter functionality of the developed nanoparticle system, as both magnetic resonance imaging (MRI) and fluorescent contrast agent. It was demonstrated that the nanoparticle system was made of an iron oxide nanoparticle (NP), with a thin covalently bound polyethylene glycol (PEG) coating, which is functionalized with peptide-MHC monomers and coupled with the fluorophore Alexa Fluor 647. Peptide-MHC monomers were used to impart targeting specificity to the NP-PEG.

Original language	English (US)
Pages (from-to)	712-715
Number of pages	4
Journal	Small
Volume	4
Issue number	6
DOIs	https://doi.org/10.1002/smll.200701103
State	Published - Jun 2008
Externally published	Yes

Keywords

Cell labeling
Labeling efficacy
Magnetic nanoparticles
Magnetic resonance imaging
Specific targeting

ASJC Scopus subject areas

Biotechnology
Biomaterials
General Chemistry
General Materials Science

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10.1002/smll.200701103

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abstract = "A study was conducted to demonstrate a dual-modality nanoparticle system, capable of labeling and imaging CTL cells, expressing T-cell receptors (TCR) that recognize cognate MHC-peptide complexes on the surface of antigen-presenting tumor cells. The study demonstrated in-vivo T-cell selectivity and reporter functionality of the developed nanoparticle system, as both magnetic resonance imaging (MRI) and fluorescent contrast agent. It was demonstrated that the nanoparticle system was made of an iron oxide nanoparticle (NP), with a thin covalently bound polyethylene glycol (PEG) coating, which is functionalized with peptide-MHC monomers and coupled with the fluorophore Alexa Fluor 647. Peptide-MHC monomers were used to impart targeting specificity to the NP-PEG.",

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AU - Gunn, Jonathan

AU - Wallen, Herschel

AU - Veiseh, Omid

AU - Sun, Conroy

AU - Fang, Chen

AU - Cao, Jianhong

AU - Yee, Cassian

AU - Zhang, Miqin

PY - 2008/6

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AB - A study was conducted to demonstrate a dual-modality nanoparticle system, capable of labeling and imaging CTL cells, expressing T-cell receptors (TCR) that recognize cognate MHC-peptide complexes on the surface of antigen-presenting tumor cells. The study demonstrated in-vivo T-cell selectivity and reporter functionality of the developed nanoparticle system, as both magnetic resonance imaging (MRI) and fluorescent contrast agent. It was demonstrated that the nanoparticle system was made of an iron oxide nanoparticle (NP), with a thin covalently bound polyethylene glycol (PEG) coating, which is functionalized with peptide-MHC monomers and coupled with the fluorophore Alexa Fluor 647. Peptide-MHC monomers were used to impart targeting specificity to the NP-PEG.

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KW - Labeling efficacy

KW - Magnetic nanoparticles

KW - Magnetic resonance imaging

KW - Specific targeting

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A multimodal targeting nanoparticle for selectively labeling T. cells

Abstract

Keywords

ASJC Scopus subject areas

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