A new murine esophageal organoid culture method and organoid-based model of esophageal squamous cell neoplasia

Biyun Zheng; Kyung Pil Ko; Xuefen Fang; Xiaozhong Wang; Jie Zhang; Sohee Jun; Bong Jun Kim; Wenyi Luo; Moon Jong Kim; Youn Sang Jung; Christopher L. Cervantes; Jae Il Park

doi:10.1016/j.isci.2021.103440

A new murine esophageal organoid culture method and organoid-based model of esophageal squamous cell neoplasia

Biyun Zheng, Kyung Pil Ko, Xuefen Fang, Xiaozhong Wang, Jie Zhang, Sohee Jun, Bong Jun Kim, Wenyi Luo, Moon Jong Kim, Youn Sang Jung, Christopher L. Cervantes, Jae Il Park

Experimental Radiation Oncology

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Organoids mimic the physiologic and pathologic events of organs. However, no consensus on esophageal organoid (EO) culture methods has been reached. Moreover, organoid models reproducing esophageal squamous cell carcinoma (ESCC) initiation have been unavailable. Herein, we sought to develop an esophageal minimum essential organoid culture medium (E-MEOM) for culturing murine EOs and establishing an early ESCC model. We formulated E-MEOM to grow EOs from a single cell with clonal expansion, maintenance, and passage. We found that EOs cultured in E-MEOM were equivalent to the esophageal epithelium by histological analysis and transcriptomic study. Trp53 knockout and Kras^G12D expression in EOs induced the development of esophageal squamous neoplasia, an early lesion of ESCC. Here we propose the new formula for EO culture with minimum components and the organoid model recapitulating ESCC initiation, laying the foundation for ESCC research and drug discovery.

Original language	English (US)
Article number	103440
Journal	iScience
Volume	24
Issue number	12
DOIs	https://doi.org/10.1016/j.isci.2021.103440
State	Published - Dec 17 2021

Keywords

Biological sciences research methodologies
Cancer
Methodology in biological science

ASJC Scopus subject areas

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title = "A new murine esophageal organoid culture method and organoid-based model of esophageal squamous cell neoplasia",

abstract = "Organoids mimic the physiologic and pathologic events of organs. However, no consensus on esophageal organoid (EO) culture methods has been reached. Moreover, organoid models reproducing esophageal squamous cell carcinoma (ESCC) initiation have been unavailable. Herein, we sought to develop an esophageal minimum essential organoid culture medium (E-MEOM) for culturing murine EOs and establishing an early ESCC model. We formulated E-MEOM to grow EOs from a single cell with clonal expansion, maintenance, and passage. We found that EOs cultured in E-MEOM were equivalent to the esophageal epithelium by histological analysis and transcriptomic study. Trp53 knockout and KrasG12D expression in EOs induced the development of esophageal squamous neoplasia, an early lesion of ESCC. Here we propose the new formula for EO culture with minimum components and the organoid model recapitulating ESCC initiation, laying the foundation for ESCC research and drug discovery.",

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AU - Zheng, Biyun

AU - Ko, Kyung Pil

AU - Fang, Xuefen

AU - Wang, Xiaozhong

AU - Zhang, Jie

AU - Jun, Sohee

AU - Kim, Bong Jun

AU - Luo, Wenyi

AU - Kim, Moon Jong

AU - Jung, Youn Sang

AU - Cervantes, Christopher L.

AU - Park, Jae Il

PY - 2021/12/17

Y1 - 2021/12/17

N2 - Organoids mimic the physiologic and pathologic events of organs. However, no consensus on esophageal organoid (EO) culture methods has been reached. Moreover, organoid models reproducing esophageal squamous cell carcinoma (ESCC) initiation have been unavailable. Herein, we sought to develop an esophageal minimum essential organoid culture medium (E-MEOM) for culturing murine EOs and establishing an early ESCC model. We formulated E-MEOM to grow EOs from a single cell with clonal expansion, maintenance, and passage. We found that EOs cultured in E-MEOM were equivalent to the esophageal epithelium by histological analysis and transcriptomic study. Trp53 knockout and KrasG12D expression in EOs induced the development of esophageal squamous neoplasia, an early lesion of ESCC. Here we propose the new formula for EO culture with minimum components and the organoid model recapitulating ESCC initiation, laying the foundation for ESCC research and drug discovery.

AB - Organoids mimic the physiologic and pathologic events of organs. However, no consensus on esophageal organoid (EO) culture methods has been reached. Moreover, organoid models reproducing esophageal squamous cell carcinoma (ESCC) initiation have been unavailable. Herein, we sought to develop an esophageal minimum essential organoid culture medium (E-MEOM) for culturing murine EOs and establishing an early ESCC model. We formulated E-MEOM to grow EOs from a single cell with clonal expansion, maintenance, and passage. We found that EOs cultured in E-MEOM were equivalent to the esophageal epithelium by histological analysis and transcriptomic study. Trp53 knockout and KrasG12D expression in EOs induced the development of esophageal squamous neoplasia, an early lesion of ESCC. Here we propose the new formula for EO culture with minimum components and the organoid model recapitulating ESCC initiation, laying the foundation for ESCC research and drug discovery.

KW - Biological sciences research methodologies

KW - Cancer

KW - Methodology in biological science

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A new murine esophageal organoid culture method and organoid-based model of esophageal squamous cell neoplasia

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