TY - JOUR
T1 - A novel four-color PCR assay to assess T-cell receptor gamma gene rearrangements in lymphoproliferative lesions
AU - Vega, Francisco
AU - Medeiros, L. Jeffrey
AU - Jones, Dan
AU - Abruzzo, Lynne V.
AU - Lai, Raymond
AU - Manning, John
AU - Dunmire, Valerie
AU - Luthra, Rajyalakshmi
PY - 2001/7
Y1 - 2001/7
N2 - We describe a novel 4-color polymerase chain reaction (PCR) assay combined with GeneScan analysis to assess for T-cell receptor gamma chain gene (TCRgamma) rearrangements and evaluate its usefulness in 86 lymphoproliferative lesions. In this assay, each variable region (V gamma) family primer is 5′ end-labeled with a different fluorescent dye, allowing determination of the V gamma family involved in each TCRgamma rearrangement. PCR products were analyzed by capillary electrophoresis. We detected clonal TCRgamma rearrangements in 60 (98%) of 61 T-cell lymphomas, 2 (15%) of 13 B-cell lymphomas, and 3 (25%) of 12 reactive lesions. These results compared favorably with conventional PCR methods using denaturing gradient gel electrophoresis, which revealed clonal TCRgamma rearrangements in 37 (90%) of 41 T-cell lymphomas, 1 (25%) of 4 B-cell lymphomas, and 2 (25%) of 8 reactive lesions. This 4-color PCR assay is at least equivalent to conventional PCR methods and is convenient, allows accurate size determination of TCRgamma rearrangements, and identifies the specific V gamma family involved, providing more specific information about TCRgamma rearrangement.
AB - We describe a novel 4-color polymerase chain reaction (PCR) assay combined with GeneScan analysis to assess for T-cell receptor gamma chain gene (TCRgamma) rearrangements and evaluate its usefulness in 86 lymphoproliferative lesions. In this assay, each variable region (V gamma) family primer is 5′ end-labeled with a different fluorescent dye, allowing determination of the V gamma family involved in each TCRgamma rearrangement. PCR products were analyzed by capillary electrophoresis. We detected clonal TCRgamma rearrangements in 60 (98%) of 61 T-cell lymphomas, 2 (15%) of 13 B-cell lymphomas, and 3 (25%) of 12 reactive lesions. These results compared favorably with conventional PCR methods using denaturing gradient gel electrophoresis, which revealed clonal TCRgamma rearrangements in 37 (90%) of 41 T-cell lymphomas, 1 (25%) of 4 B-cell lymphomas, and 2 (25%) of 8 reactive lesions. This 4-color PCR assay is at least equivalent to conventional PCR methods and is convenient, allows accurate size determination of TCRgamma rearrangements, and identifies the specific V gamma family involved, providing more specific information about TCRgamma rearrangement.
KW - 4-color TCRgamma PCR assay
KW - High-resolution capillary electrophoresis
KW - Polymerase chain reaction
KW - T-cell lymphoma
KW - T-cell receptor gamma chain gene
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U2 - 10.1309/5WFQ-N12E-DT05-UX1T
DO - 10.1309/5WFQ-N12E-DT05-UX1T
M3 - Article
C2 - 11447747
AN - SCOPUS:0034950512
SN - 0002-9173
VL - 116
SP - 17
EP - 24
JO - American journal of clinical pathology
JF - American journal of clinical pathology
IS - 1
ER -