A novel gene encoding a B-box protein within the BRCA1 region at 17q21.1

Ian G. Campbell; Hans M. Nicolal; William D. Foulkes; Abriel Senger; Gordon W. Stamps; Gordon Allans; Clnda Boyer; Karen Jones; Robert C. Bast; Ellen Solomon; John Trowsdale; Donald M. Black

doi:10.1093/hmg/3.4.589

A novel gene encoding a B-box protein within the BRCA1 region at 17q21.1

Ian G. Campbell, Hans M. Nicolal, William D. Foulkes, Abriel Senger, Gordon W. Stamps, Gordon Allans, Clnda Boyer, Karen Jones, Robert C. Bast, Ellen Solomon, John Trowsdale, Donald M. Black

Research output: Contribution to journal › Article › peer-review

108 Scopus citations

Abstract

A novel cDNA clone was Isolated using a polyclonal serum directed against partially purified ovarian carcinoma antigen CA125. The deduced peptide sequence lacked membrane protein characteristics expected for CA125 but encompassed a B-box/coiled coll motif present in many genes with transformation potential. The gene was mapped by fluorescence In situ hybridization within the minimum region known to contain the familial breast/ovarian carcinoma gene, BRCA1. YAC and cosmid clones were isolated and used to refine the location of this gene adjacent and proximal to the RNU2 locus. The exon structure of the gene was determined. Extensive SSCP and sequence analysis of over 100 tumour and normal DNAs from familial and sporadic breast cancers and sporadic ovarian cancers failed to detect mutations In the coding region of this gene.

Original language	English (US)
Pages (from-to)	589-594
Number of pages	6
Journal	Human molecular genetics
Volume	3
Issue number	4
DOIs	https://doi.org/10.1093/hmg/3.4.589
State	Published - Apr 1994
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology
Genetics
Genetics(clinical)

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title = "A novel gene encoding a B-box protein within the BRCA1 region at 17q21.1",

abstract = "A novel cDNA clone was Isolated using a polyclonal serum directed against partially purified ovarian carcinoma antigen CA125. The deduced peptide sequence lacked membrane protein characteristics expected for CA125 but encompassed a B-box/coiled coll motif present in many genes with transformation potential. The gene was mapped by fluorescence In situ hybridization within the minimum region known to contain the familial breast/ovarian carcinoma gene, BRCA1. YAC and cosmid clones were isolated and used to refine the location of this gene adjacent and proximal to the RNU2 locus. The exon structure of the gene was determined. Extensive SSCP and sequence analysis of over 100 tumour and normal DNAs from familial and sporadic breast cancers and sporadic ovarian cancers failed to detect mutations In the coding region of this gene.",

author = "Campbell, {Ian G.} and Nicolal, {Hans M.} and Foulkes, {William D.} and Abriel Senger and Stamps, {Gordon W.} and Gordon Allans and Clnda Boyer and Karen Jones and Bast, {Robert C.} and Ellen Solomon and John Trowsdale and Black, {Donald M.}",

note = "Funding Information: GWHS acknowledges the support of the Association of International Cancer Research. The authors wish to thank Paul Freemont for his assistance with the sequence analysis.",

year = "1994",

month = apr,

doi = "10.1093/hmg/3.4.589",

language = "English (US)",

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pages = "589--594",

journal = "Human molecular genetics",

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publisher = "Oxford University Press",

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AU - Campbell, Ian G.

AU - Nicolal, Hans M.

AU - Foulkes, William D.

AU - Senger, Abriel

AU - Stamps, Gordon W.

AU - Allans, Gordon

AU - Boyer, Clnda

AU - Jones, Karen

AU - Bast, Robert C.

AU - Solomon, Ellen

AU - Trowsdale, John

AU - Black, Donald M.

N1 - Funding Information: GWHS acknowledges the support of the Association of International Cancer Research. The authors wish to thank Paul Freemont for his assistance with the sequence analysis.

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N2 - A novel cDNA clone was Isolated using a polyclonal serum directed against partially purified ovarian carcinoma antigen CA125. The deduced peptide sequence lacked membrane protein characteristics expected for CA125 but encompassed a B-box/coiled coll motif present in many genes with transformation potential. The gene was mapped by fluorescence In situ hybridization within the minimum region known to contain the familial breast/ovarian carcinoma gene, BRCA1. YAC and cosmid clones were isolated and used to refine the location of this gene adjacent and proximal to the RNU2 locus. The exon structure of the gene was determined. Extensive SSCP and sequence analysis of over 100 tumour and normal DNAs from familial and sporadic breast cancers and sporadic ovarian cancers failed to detect mutations In the coding region of this gene.

AB - A novel cDNA clone was Isolated using a polyclonal serum directed against partially purified ovarian carcinoma antigen CA125. The deduced peptide sequence lacked membrane protein characteristics expected for CA125 but encompassed a B-box/coiled coll motif present in many genes with transformation potential. The gene was mapped by fluorescence In situ hybridization within the minimum region known to contain the familial breast/ovarian carcinoma gene, BRCA1. YAC and cosmid clones were isolated and used to refine the location of this gene adjacent and proximal to the RNU2 locus. The exon structure of the gene was determined. Extensive SSCP and sequence analysis of over 100 tumour and normal DNAs from familial and sporadic breast cancers and sporadic ovarian cancers failed to detect mutations In the coding region of this gene.

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A novel gene encoding a B-box protein within the BRCA1 region at 17q21.1

Abstract

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