@inbook{2b76061f1ee54036ab6a037798fc7169,
title = "A Novel Nonviral Gene Delivery System: Multifunctional Envelope-Type Nano Device",
abstract = "In this review we introduce a new concept for developing a nonviral gene delivery system which we call {"}Programmed Packaging.{"} Based on this concept, we succeeded in developing a multifunctional envelope-type nano device (MEND), which exerts high transfection activities equivalent to those of an adenovirus in a dividing cell. The use of MEND has been extended to in vivo applications. PEG/ peptide/DOPE ternary conjugate (PPD)-MEND, a new in vivo gene delivery system for the targeting of tumor cells that dissociates surface-modified PEG in tumor tissue by matrix metalloproteinase (MMP) and exerts significant transfection activities, was developed. In parallel with the development of MEND, a quantitative gene delivery system, Confocal Image-assisted 3-dimensionally integrated quantification (CIDIQ), also was developed. This method identified the rate-limiting step of the nonviral gene delivery system by comparing it with adenoviral-mediated gene delivery. The results of this analysis provide a new direction for the development of rational nonviral gene delivery systems.",
keywords = "Gene delivery, Intracellular trafficking, MEND, Nano device, Nonviral",
author = "Hiroto Hatakeyama and Hidetaka Akita and Kentaro Kogure and Hideyoshi Harashima",
note = "Funding Information: This study was performed through Special Coordination Funds for Promoting Science and Technology of the Ministry of Education, Culture, Sports, Science and Technology, the Japanese Government. The authors also wish to thank Dr. James L. McDonald for his helpful advice in writing the English manuscript.",
year = "2010",
doi = "10.1007/10_2008_40",
language = "English (US)",
isbn = "9783642149467",
series = "Advances in Biochemical Engineering/Biotechnology",
pages = "197--230",
booktitle = "Nano/Micro Biotechnology",
}