A novel role of EMMPRIN/CD147 in transformation of quiescent fibroblasts to cancer-associated fibroblasts by breast cancer cells

Jing Xu; Yang Lu; Songbo Qiu; Zhi Nan Chen; Zhen Fan

doi:10.1016/j.canlet.2013.02.054

A novel role of EMMPRIN/CD147 in transformation of quiescent fibroblasts to cancer-associated fibroblasts by breast cancer cells

Jing Xu, Yang Lu, Songbo Qiu, Zhi Nan Chen, Zhen Fan

Experimental Therapeutics

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

We tested the novel hypothesis that EMMPRIN/CD147, a transmembrane glycoprotein overexpressed in breast cancer cells, has a previously unknown role in transforming fibroblasts to cancer-associated fibroblasts, and that cancer-associated fibroblasts in turn induce epithelial-to-mesenchymal transition of breast cancer cells. Co-culture of fibroblasts with breast cancer cells or treatment of fibroblasts with breast cancer cell conditioned culture medium or recombinant EMMPRIN/CD147 induced expression of α-SMA in the fibroblasts in an EMMPRIN/CD147-dependent manner and promoted epithelial-to-mesenchymal transition of breast cancer cells and enhanced cell migration potential. These findings support a novel role of EMMPRIN/CD147 in regulating the interaction between cancer and stroma.

Original language	English (US)
Pages (from-to)	380-386
Number of pages	7
Journal	Cancer Letters
Volume	335
Issue number	2
DOIs	https://doi.org/10.1016/j.canlet.2013.02.054
State	Published - Jul 28 2013

Keywords

Cancer stroma
Cancer-associated fibroblasts
EMMPRIN/CD147
Epithelial-to-mesenchymal transition
α-SMA

ASJC Scopus subject areas

Oncology
Cancer Research

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10.1016/j.canlet.2013.02.054

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title = "A novel role of EMMPRIN/CD147 in transformation of quiescent fibroblasts to cancer-associated fibroblasts by breast cancer cells",

abstract = "We tested the novel hypothesis that EMMPRIN/CD147, a transmembrane glycoprotein overexpressed in breast cancer cells, has a previously unknown role in transforming fibroblasts to cancer-associated fibroblasts, and that cancer-associated fibroblasts in turn induce epithelial-to-mesenchymal transition of breast cancer cells. Co-culture of fibroblasts with breast cancer cells or treatment of fibroblasts with breast cancer cell conditioned culture medium or recombinant EMMPRIN/CD147 induced expression of α-SMA in the fibroblasts in an EMMPRIN/CD147-dependent manner and promoted epithelial-to-mesenchymal transition of breast cancer cells and enhanced cell migration potential. These findings support a novel role of EMMPRIN/CD147 in regulating the interaction between cancer and stroma.",

keywords = "Cancer stroma, Cancer-associated fibroblasts, EMMPRIN/CD147, Epithelial-to-mesenchymal transition, α-SMA",

author = "Jing Xu and Yang Lu and Songbo Qiu and Chen, {Zhi Nan} and Zhen Fan",

note = "Funding Information: This work was supported in part by US Department of Defense Congressionally Directed Medical Research Program award W81XWH-11-1-0107 (to Z.F.), a Breast Cancer Research Foundation award (to Z.F.), China National Basic Research Program (the 973 Program) award 2009CB521704 (to Z-N.C.), and China National Natural Science Foundation award 30800573 (to J.X.). The work was also supported in part by the NIH through MD Anderson{\textquoteright}s Cancer Center Support Grant, CA016672. We thank Stephanie Deming of the Department of Scientific Publications at The University of Texas MD Anderson Cancer Center for editorial assistance.",

year = "2013",

month = jul,

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doi = "10.1016/j.canlet.2013.02.054",

language = "English (US)",

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pages = "380--386",

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AU - Xu, Jing

AU - Lu, Yang

AU - Qiu, Songbo

AU - Chen, Zhi Nan

AU - Fan, Zhen

N1 - Funding Information: This work was supported in part by US Department of Defense Congressionally Directed Medical Research Program award W81XWH-11-1-0107 (to Z.F.), a Breast Cancer Research Foundation award (to Z.F.), China National Basic Research Program (the 973 Program) award 2009CB521704 (to Z-N.C.), and China National Natural Science Foundation award 30800573 (to J.X.). The work was also supported in part by the NIH through MD Anderson’s Cancer Center Support Grant, CA016672. We thank Stephanie Deming of the Department of Scientific Publications at The University of Texas MD Anderson Cancer Center for editorial assistance.

PY - 2013/7/28

Y1 - 2013/7/28

N2 - We tested the novel hypothesis that EMMPRIN/CD147, a transmembrane glycoprotein overexpressed in breast cancer cells, has a previously unknown role in transforming fibroblasts to cancer-associated fibroblasts, and that cancer-associated fibroblasts in turn induce epithelial-to-mesenchymal transition of breast cancer cells. Co-culture of fibroblasts with breast cancer cells or treatment of fibroblasts with breast cancer cell conditioned culture medium or recombinant EMMPRIN/CD147 induced expression of α-SMA in the fibroblasts in an EMMPRIN/CD147-dependent manner and promoted epithelial-to-mesenchymal transition of breast cancer cells and enhanced cell migration potential. These findings support a novel role of EMMPRIN/CD147 in regulating the interaction between cancer and stroma.

AB - We tested the novel hypothesis that EMMPRIN/CD147, a transmembrane glycoprotein overexpressed in breast cancer cells, has a previously unknown role in transforming fibroblasts to cancer-associated fibroblasts, and that cancer-associated fibroblasts in turn induce epithelial-to-mesenchymal transition of breast cancer cells. Co-culture of fibroblasts with breast cancer cells or treatment of fibroblasts with breast cancer cell conditioned culture medium or recombinant EMMPRIN/CD147 induced expression of α-SMA in the fibroblasts in an EMMPRIN/CD147-dependent manner and promoted epithelial-to-mesenchymal transition of breast cancer cells and enhanced cell migration potential. These findings support a novel role of EMMPRIN/CD147 in regulating the interaction between cancer and stroma.

KW - Cancer stroma

KW - Cancer-associated fibroblasts

KW - EMMPRIN/CD147

KW - Epithelial-to-mesenchymal transition

KW - α-SMA

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A novel role of EMMPRIN/CD147 in transformation of quiescent fibroblasts to cancer-associated fibroblasts by breast cancer cells

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Keywords

ASJC Scopus subject areas

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