A packaging system for SV40 vectors without vital coding sequences

Bingliang Fang; Patricia Koch; Michael Bouvet; Lin Ji; Jack A. Roth

doi:10.1006/abio.1997.2417

A packaging system for SV40 vectors without vital coding sequences

Bingliang Fang, Patricia Koch, Michael Bouvet, Lin Ji, Jack A. Roth

Thoracic & Cardiovascular Surgery

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11 Scopus citations

Abstract

SV40 vectors have been used as expression vectors for mammalian cells since the early 1980s. More recently, they have been used as gene transfer vectors in mice and in human peripheral blood cells. Here we described a system for packaging SV40 vectors without viral coding sequences. Recombinant adenovirus-expressing SV40 capsids can effectively package plasmids that contain the SV40 replication origin. The final yield of infectious SV40 vector is about 3 x 10⁵, with a SV40 to adenoviral vector ratio of about 1000:1. Helper adenoviruses can be effectively heat-inactivated with no effect on the infectivity of SV40 vectors.

Original language	English (US)
Pages (from-to)	139-143
Number of pages	5
Journal	Analytical Biochemistry
Volume	254
Issue number	1
DOIs	https://doi.org/10.1006/abio.1997.2417
State	Published - Dec 1 1997

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1006/abio.1997.2417

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title = "A packaging system for SV40 vectors without vital coding sequences",

abstract = "SV40 vectors have been used as expression vectors for mammalian cells since the early 1980s. More recently, they have been used as gene transfer vectors in mice and in human peripheral blood cells. Here we described a system for packaging SV40 vectors without viral coding sequences. Recombinant adenovirus-expressing SV40 capsids can effectively package plasmids that contain the SV40 replication origin. The final yield of infectious SV40 vector is about 3 x 105, with a SV40 to adenoviral vector ratio of about 1000:1. Helper adenoviruses can be effectively heat-inactivated with no effect on the infectivity of SV40 vectors.",

author = "Bingliang Fang and Patricia Koch and Michael Bouvet and Lin Ji and Roth, {Jack A.}",

note = "Funding Information: We thank Dr. J. S. Butel for providing us the anti-SV40 antiserum used in this study, Jude Richard for editorial review, and Monica Contreras for assistance in preparing the manuscript. This study was funded by a grant from the University of Texas Physicians Referral Service, a Specialized Program of Research Excellence (SPORE) in Lung Cancer grant (P50-CA70907), and a sponsored research agreement with Introgen Therapeutics, Inc. (Austin, TX).",

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doi = "10.1006/abio.1997.2417",

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AU - Koch, Patricia

AU - Bouvet, Michael

AU - Ji, Lin

AU - Roth, Jack A.

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PY - 1997/12/1

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N2 - SV40 vectors have been used as expression vectors for mammalian cells since the early 1980s. More recently, they have been used as gene transfer vectors in mice and in human peripheral blood cells. Here we described a system for packaging SV40 vectors without viral coding sequences. Recombinant adenovirus-expressing SV40 capsids can effectively package plasmids that contain the SV40 replication origin. The final yield of infectious SV40 vector is about 3 x 105, with a SV40 to adenoviral vector ratio of about 1000:1. Helper adenoviruses can be effectively heat-inactivated with no effect on the infectivity of SV40 vectors.

AB - SV40 vectors have been used as expression vectors for mammalian cells since the early 1980s. More recently, they have been used as gene transfer vectors in mice and in human peripheral blood cells. Here we described a system for packaging SV40 vectors without viral coding sequences. Recombinant adenovirus-expressing SV40 capsids can effectively package plasmids that contain the SV40 replication origin. The final yield of infectious SV40 vector is about 3 x 105, with a SV40 to adenoviral vector ratio of about 1000:1. Helper adenoviruses can be effectively heat-inactivated with no effect on the infectivity of SV40 vectors.

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A packaging system for SV40 vectors without vital coding sequences

Abstract

ASJC Scopus subject areas

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