A simple and sensitive method for detecting adenovirus in serum and urine

Ying C. Henderson; Ta Jen Liu; Gary L. Clayman

doi:10.1016/S0166-0934(97)00189-4

A simple and sensitive method for detecting adenovirus in serum and urine

Ying C. Henderson, Ta Jen Liu, Gary L. Clayman

Head & Neck Surgery

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Abstract

A method to detect shed virus in patients' serum and urine was developed following Ad5CMV-p53 gene transfer via direct tumor injections. The procedure differs from those reported previously in that it first uses polyethylene glycol to precipitate adenoviral particles from patient serum or urine. Adenoviral DNA is then extracted following proteinase K digestion. Finally, polymerase chain reaction (PCR) amplification followed by Southern blot transfer are employed to enhance the limit of detection to less than ten viral particles. The detection limit in a 0.25-ml sample is five viral particles in serum and one vital particle in urine. This procedure is sensitive, reproducible, and can be completed in less than 2 days.

Original language	English (US)
Pages (from-to)	51-56
Number of pages	6
Journal	Journal of Virological Methods
Volume	71
Issue number	1
DOIs	https://doi.org/10.1016/S0166-0934(97)00189-4
State	Published - Mar 1998

Keywords

Adenovirus
Polyethylene glycol
Serum
Urine

ASJC Scopus subject areas

Virology

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10.1016/S0166-0934(97)00189-4

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title = "A simple and sensitive method for detecting adenovirus in serum and urine",

abstract = "A method to detect shed virus in patients' serum and urine was developed following Ad5CMV-p53 gene transfer via direct tumor injections. The procedure differs from those reported previously in that it first uses polyethylene glycol to precipitate adenoviral particles from patient serum or urine. Adenoviral DNA is then extracted following proteinase K digestion. Finally, polymerase chain reaction (PCR) amplification followed by Southern blot transfer are employed to enhance the limit of detection to less than ten viral particles. The detection limit in a 0.25-ml sample is five viral particles in serum and one vital particle in urine. This procedure is sensitive, reproducible, and can be completed in less than 2 days.",

keywords = "Adenovirus, Polyethylene glycol, Serum, Urine",

author = "Henderson, {Ying C.} and Liu, {Ta Jen} and Clayman, {Gary L.}",

note = "Funding Information: This work was supported in part by a NIH R29 grant to Dr Gary Clayman, The University of Texas M.D. Anderson Cancer Center Core Grant CA-16672, and a sponsored research agreement with Introgen Therapeutics. We thank Mary Wang for preparing the Ad5CMV-p53 virus, Lore Feldman for editing the manuscript, Dr Dennis A. Johnston for a helpful discussion of relevant statistical issues, Dr J. Michael Hudson for critical reading of the manuscript, and Lillian K. Meander for editorial assistance. ",

year = "1998",

month = mar,

doi = "10.1016/S0166-0934(97)00189-4",

language = "English (US)",

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pages = "51--56",

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T1 - A simple and sensitive method for detecting adenovirus in serum and urine

AU - Henderson, Ying C.

AU - Liu, Ta Jen

AU - Clayman, Gary L.

N1 - Funding Information: This work was supported in part by a NIH R29 grant to Dr Gary Clayman, The University of Texas M.D. Anderson Cancer Center Core Grant CA-16672, and a sponsored research agreement with Introgen Therapeutics. We thank Mary Wang for preparing the Ad5CMV-p53 virus, Lore Feldman for editing the manuscript, Dr Dennis A. Johnston for a helpful discussion of relevant statistical issues, Dr J. Michael Hudson for critical reading of the manuscript, and Lillian K. Meander for editorial assistance.

PY - 1998/3

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N2 - A method to detect shed virus in patients' serum and urine was developed following Ad5CMV-p53 gene transfer via direct tumor injections. The procedure differs from those reported previously in that it first uses polyethylene glycol to precipitate adenoviral particles from patient serum or urine. Adenoviral DNA is then extracted following proteinase K digestion. Finally, polymerase chain reaction (PCR) amplification followed by Southern blot transfer are employed to enhance the limit of detection to less than ten viral particles. The detection limit in a 0.25-ml sample is five viral particles in serum and one vital particle in urine. This procedure is sensitive, reproducible, and can be completed in less than 2 days.

AB - A method to detect shed virus in patients' serum and urine was developed following Ad5CMV-p53 gene transfer via direct tumor injections. The procedure differs from those reported previously in that it first uses polyethylene glycol to precipitate adenoviral particles from patient serum or urine. Adenoviral DNA is then extracted following proteinase K digestion. Finally, polymerase chain reaction (PCR) amplification followed by Southern blot transfer are employed to enhance the limit of detection to less than ten viral particles. The detection limit in a 0.25-ml sample is five viral particles in serum and one vital particle in urine. This procedure is sensitive, reproducible, and can be completed in less than 2 days.

KW - Adenovirus

KW - Polyethylene glycol

KW - Serum

KW - Urine

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JO - Journal of Virological Methods

JF - Journal of Virological Methods

IS - 1

ER -

A simple and sensitive method for detecting adenovirus in serum and urine

Abstract

Keywords

ASJC Scopus subject areas

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