A strategy for enhancing the engraftment of human hematopoietic stem cells in NOD/SCID mice

Soo Hyun Lee; Dae Seong Kim; Myoung Woo Lee; Yoo Hun Noh; In Keun Jang; Dong Hyun Kim; Heung Mo Yang; Sung Joo Kim; Soo Jin Choi; Wonil Oh; Yoon Sun Yang; Hee Won Chueh; Meong Hi Son; Hye Lim Jung; Keon Hee Yoo; Ki Woong Sung; Hong Hoe Koo

doi:10.1007/s00277-013-1830-1

A strategy for enhancing the engraftment of human hematopoietic stem cells in NOD/SCID mice

Soo Hyun Lee, Dae Seong Kim, Myoung Woo Lee, Yoo Hun Noh, In Keun Jang, Dong Hyun Kim, Heung Mo Yang, Sung Joo Kim, Soo Jin Choi, Wonil Oh, Yoon Sun Yang, Hee Won Chueh, Meong Hi Son, Hye Lim Jung, Keon Hee Yoo, Ki Woong Sung, Hong Hoe Koo

Pathology

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

To overcome the limitations of allogeneic hematopoietic stem cell transplantation (HSCT), we conducted a study to identify a strategy for enhancing hematopoietic stem cell (HSC) engraftment during HSCT. Co-transplantation experiments with mesenchymal stem cells (MSCs) derived from adult human tissues including bone marrow (BM), adipose tissue (AT), and umbilical cord blood (CB) were conducted. We showed that AT-MSCs and CB-MSCs enhanced the engraftment of HSCs as effectively as BM-MSCs in NOD/SCID mice, suggesting that AT-MSCs and CB-MSCs can be used as alternative stem cell sources for enhancing the engraftment and homing of HSCs. CB-MSCs derived from different donors showed different degrees of efficacy in enhancing the engraftment of HSCs. The most effective CB-MSCs showed higher proliferation rates and secreted more MCP-1, RANTES, EGF, and VEGF. Our results suggest that AT-MSCs and CB-MSCs could be alternative stem cell sources for co-transplantation in HSCT. Furthermore, in terms of MSCs' heterogeneity, characteristics of each population of MSCs are considerable factors for selecting MSCs suitable for co-transplantation with HSC.

Original language	English (US)
Pages (from-to)	1595-1602
Number of pages	8
Journal	Annals of Hematology
Volume	92
Issue number	12
DOIs	https://doi.org/10.1007/s00277-013-1830-1
State	Published - Dec 2013

Keywords

Co-transplantation
Engraftment
Hematopoietic stem cell transplantation
Heterogeneity
Mesenchymal stem cell

ASJC Scopus subject areas

Hematology

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10.1007/s00277-013-1830-1

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Lee, S. H., Kim, D. S., Lee, M. W., Noh, Y. H., Jang, I. K., Kim, D. H., Yang, H. M., Kim, S. J., Choi, S. J., Oh, W., Yang, Y. S., Chueh, H. W., Son, M. H., Jung, H. L., Yoo, K. H., Sung, K. W., & Koo, H. H. (2013). A strategy for enhancing the engraftment of human hematopoietic stem cells in NOD/SCID mice. Annals of Hematology, 92(12), 1595-1602. https://doi.org/10.1007/s00277-013-1830-1

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title = "A strategy for enhancing the engraftment of human hematopoietic stem cells in NOD/SCID mice",

abstract = "To overcome the limitations of allogeneic hematopoietic stem cell transplantation (HSCT), we conducted a study to identify a strategy for enhancing hematopoietic stem cell (HSC) engraftment during HSCT. Co-transplantation experiments with mesenchymal stem cells (MSCs) derived from adult human tissues including bone marrow (BM), adipose tissue (AT), and umbilical cord blood (CB) were conducted. We showed that AT-MSCs and CB-MSCs enhanced the engraftment of HSCs as effectively as BM-MSCs in NOD/SCID mice, suggesting that AT-MSCs and CB-MSCs can be used as alternative stem cell sources for enhancing the engraftment and homing of HSCs. CB-MSCs derived from different donors showed different degrees of efficacy in enhancing the engraftment of HSCs. The most effective CB-MSCs showed higher proliferation rates and secreted more MCP-1, RANTES, EGF, and VEGF. Our results suggest that AT-MSCs and CB-MSCs could be alternative stem cell sources for co-transplantation in HSCT. Furthermore, in terms of MSCs' heterogeneity, characteristics of each population of MSCs are considerable factors for selecting MSCs suitable for co-transplantation with HSC.",

keywords = "Co-transplantation, Engraftment, Hematopoietic stem cell transplantation, Heterogeneity, Mesenchymal stem cell",

author = "Lee, {Soo Hyun} and Kim, {Dae Seong} and Lee, {Myoung Woo} and Noh, {Yoo Hun} and Jang, {In Keun} and Kim, {Dong Hyun} and Yang, {Heung Mo} and Kim, {Sung Joo} and Choi, {Soo Jin} and Wonil Oh and Yang, {Yoon Sun} and Chueh, {Hee Won} and Son, {Meong Hi} and Jung, {Hye Lim} and Yoo, {Keon Hee} and Sung, {Ki Woong} and Koo, {Hong Hoe}",

note = "Funding Information: Acknowledgments This study was supported by a grant of the Korean Health Technology R&D Project, Ministry for Health, Welfare and Family affairs, Republic of Korea (project no: A090729).",

year = "2013",

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doi = "10.1007/s00277-013-1830-1",

language = "English (US)",

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T1 - A strategy for enhancing the engraftment of human hematopoietic stem cells in NOD/SCID mice

AU - Lee, Soo Hyun

AU - Kim, Dae Seong

AU - Lee, Myoung Woo

AU - Noh, Yoo Hun

AU - Jang, In Keun

AU - Kim, Dong Hyun

AU - Yang, Heung Mo

AU - Kim, Sung Joo

AU - Choi, Soo Jin

AU - Oh, Wonil

AU - Yang, Yoon Sun

AU - Chueh, Hee Won

AU - Son, Meong Hi

AU - Jung, Hye Lim

AU - Yoo, Keon Hee

AU - Sung, Ki Woong

AU - Koo, Hong Hoe

N1 - Funding Information: Acknowledgments This study was supported by a grant of the Korean Health Technology R&D Project, Ministry for Health, Welfare and Family affairs, Republic of Korea (project no: A090729).

PY - 2013/12

Y1 - 2013/12

N2 - To overcome the limitations of allogeneic hematopoietic stem cell transplantation (HSCT), we conducted a study to identify a strategy for enhancing hematopoietic stem cell (HSC) engraftment during HSCT. Co-transplantation experiments with mesenchymal stem cells (MSCs) derived from adult human tissues including bone marrow (BM), adipose tissue (AT), and umbilical cord blood (CB) were conducted. We showed that AT-MSCs and CB-MSCs enhanced the engraftment of HSCs as effectively as BM-MSCs in NOD/SCID mice, suggesting that AT-MSCs and CB-MSCs can be used as alternative stem cell sources for enhancing the engraftment and homing of HSCs. CB-MSCs derived from different donors showed different degrees of efficacy in enhancing the engraftment of HSCs. The most effective CB-MSCs showed higher proliferation rates and secreted more MCP-1, RANTES, EGF, and VEGF. Our results suggest that AT-MSCs and CB-MSCs could be alternative stem cell sources for co-transplantation in HSCT. Furthermore, in terms of MSCs' heterogeneity, characteristics of each population of MSCs are considerable factors for selecting MSCs suitable for co-transplantation with HSC.

AB - To overcome the limitations of allogeneic hematopoietic stem cell transplantation (HSCT), we conducted a study to identify a strategy for enhancing hematopoietic stem cell (HSC) engraftment during HSCT. Co-transplantation experiments with mesenchymal stem cells (MSCs) derived from adult human tissues including bone marrow (BM), adipose tissue (AT), and umbilical cord blood (CB) were conducted. We showed that AT-MSCs and CB-MSCs enhanced the engraftment of HSCs as effectively as BM-MSCs in NOD/SCID mice, suggesting that AT-MSCs and CB-MSCs can be used as alternative stem cell sources for enhancing the engraftment and homing of HSCs. CB-MSCs derived from different donors showed different degrees of efficacy in enhancing the engraftment of HSCs. The most effective CB-MSCs showed higher proliferation rates and secreted more MCP-1, RANTES, EGF, and VEGF. Our results suggest that AT-MSCs and CB-MSCs could be alternative stem cell sources for co-transplantation in HSCT. Furthermore, in terms of MSCs' heterogeneity, characteristics of each population of MSCs are considerable factors for selecting MSCs suitable for co-transplantation with HSC.

KW - Co-transplantation

KW - Engraftment

KW - Hematopoietic stem cell transplantation

KW - Heterogeneity

KW - Mesenchymal stem cell

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JO - Annals of Hematology

JF - Annals of Hematology

IS - 12

ER -

A strategy for enhancing the engraftment of human hematopoietic stem cells in NOD/SCID mice

Abstract

Keywords

ASJC Scopus subject areas

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