TY - JOUR
T1 - A subset of T cell receptors associated with L3T4 molecules mediates C6VL leukemia cell binding of its cognate retrovirus
AU - O'Neill, Helen C.
AU - McGrath, Michael S.
AU - Allison, James P.
AU - Weissman, Irving L.
N1 - Funding Information:
We thank J. Mason for exceptional administrative assistance and L. Jerabek and L. Hu for superb technical assistance. This work was supported by grants CA 32031 and CA 42551 from the National Cancer Institute, USPHS (to I. L. W.), CA 40041 (to J. P. A.); H. C. 0. was supported by a C. J. Martin Fellowship from the National Health and Medical Research Council of Australia. M. S. M. was supported by VA Merit Review Grant 365/6016.001 (016) CP103.
PY - 1987/4/10
Y1 - 1987/4/10
N2 - We show here that the interaction of a radiation leukemia virus-induced thymoma, C6VL, with its cognate retroviruses occurs in the vicinity of the T cell receptor (TCR). While an anti-clonotypic antibody completely inhibits this interaction, antibodies specific for another T cell receptor complex determinant, L3T4, only partially inhibit the cell-retrovirus interaction. Several antibodies to more abundant cell-surface determinants (T200, Ly15, H-2Db) do not inhibit this interaction. Under capping conditions, either of the antibodies to L3T4 or TCR epitopes modulate virus binding receptors from the surface of C6VL/1 cells. L3T4 and the TCR do not comodulate significantly on the surface of C6VL/1 cells. These experimental findings implicate the existence of rare TCR-L3T4 complexes on C6VL/1 cells, and the involvement of these complexes in the binding of C6VL/1 to its cognate retrovirus. In addition, the clonotypic anti-TCR antibody inhibits C6VL/1 cell proliferation at concentrations that block its binding to its produced retroviruses.
AB - We show here that the interaction of a radiation leukemia virus-induced thymoma, C6VL, with its cognate retroviruses occurs in the vicinity of the T cell receptor (TCR). While an anti-clonotypic antibody completely inhibits this interaction, antibodies specific for another T cell receptor complex determinant, L3T4, only partially inhibit the cell-retrovirus interaction. Several antibodies to more abundant cell-surface determinants (T200, Ly15, H-2Db) do not inhibit this interaction. Under capping conditions, either of the antibodies to L3T4 or TCR epitopes modulate virus binding receptors from the surface of C6VL/1 cells. L3T4 and the TCR do not comodulate significantly on the surface of C6VL/1 cells. These experimental findings implicate the existence of rare TCR-L3T4 complexes on C6VL/1 cells, and the involvement of these complexes in the binding of C6VL/1 to its cognate retrovirus. In addition, the clonotypic anti-TCR antibody inhibits C6VL/1 cell proliferation at concentrations that block its binding to its produced retroviruses.
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U2 - 10.1016/0092-8674(87)90764-1
DO - 10.1016/0092-8674(87)90764-1
M3 - Article
C2 - 2435413
AN - SCOPUS:0023649591
SN - 0092-8674
VL - 49
SP - 143
EP - 151
JO - Cell
JF - Cell
IS - 1
ER -