A triggering structure recognized by G7 monoclonal antibody on porcine lymphocytes and granulocytes

Mark E. Dato; William G. Wierda; Yoon B. Kim

doi:10.1016/0008-8749(92)90212-8

A triggering structure recognized by G7 monoclonal antibody on porcine lymphocytes and granulocytes

Mark E. Dato, William G. Wierda, Yoon B. Kim

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

Monoclonal antibody (mAb) G7 has been developed and appears to recognize a triggering structure on porcine natural killer (NK) cells and granulocytes. G7 mAb binds to approximately 13% of lymphocytes, 70% of monocytes, and >95% of granulocytes. G7 mAb does not react with B cells. G7 mAb immunoprecipitates a heterodispersed molecule of ~40 kDa. Functionally, whole but not F(ab′)₂ fragments of G7 mAb enhance NK killing of Fc receptor positive K562, U937, and MOLT-4 targets but not Fc receptor negative CEM, WEHI-164, or YAC-1 targets. Both whole and F(ab′)₂ fragments of G7 mAb inhibit lymphocyte-mediated antibody-dependent cellular cytotoxicity. Interestingly, G7 mAb induces dramatic levels of granulocyte killing against nucleated K562 targets. These results suggest that G7 mAb recognizes a trigger molecule involved in porcine cellular cytotoxicity.

Original language	English (US)
Pages (from-to)	468-477
Number of pages	10
Journal	Cellular Immunology
Volume	140
Issue number	2
DOIs	https://doi.org/10.1016/0008-8749(92)90212-8
State	Published - Apr 1 1992
Externally published	Yes

ASJC Scopus subject areas

Immunology

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10.1016/0008-8749(92)90212-8

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title = "A triggering structure recognized by G7 monoclonal antibody on porcine lymphocytes and granulocytes",

abstract = "Monoclonal antibody (mAb) G7 has been developed and appears to recognize a triggering structure on porcine natural killer (NK) cells and granulocytes. G7 mAb binds to approximately 13% of lymphocytes, 70% of monocytes, and >95% of granulocytes. G7 mAb does not react with B cells. G7 mAb immunoprecipitates a heterodispersed molecule of ~40 kDa. Functionally, whole but not F(ab′)2 fragments of G7 mAb enhance NK killing of Fc receptor positive K562, U937, and MOLT-4 targets but not Fc receptor negative CEM, WEHI-164, or YAC-1 targets. Both whole and F(ab′)2 fragments of G7 mAb inhibit lymphocyte-mediated antibody-dependent cellular cytotoxicity. Interestingly, G7 mAb induces dramatic levels of granulocyte killing against nucleated K562 targets. These results suggest that G7 mAb recognizes a trigger molecule involved in porcine cellular cytotoxicity.",

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AU - Dato, Mark E.

AU - Wierda, William G.

AU - Kim, Yoon B.

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N2 - Monoclonal antibody (mAb) G7 has been developed and appears to recognize a triggering structure on porcine natural killer (NK) cells and granulocytes. G7 mAb binds to approximately 13% of lymphocytes, 70% of monocytes, and >95% of granulocytes. G7 mAb does not react with B cells. G7 mAb immunoprecipitates a heterodispersed molecule of ~40 kDa. Functionally, whole but not F(ab′)2 fragments of G7 mAb enhance NK killing of Fc receptor positive K562, U937, and MOLT-4 targets but not Fc receptor negative CEM, WEHI-164, or YAC-1 targets. Both whole and F(ab′)2 fragments of G7 mAb inhibit lymphocyte-mediated antibody-dependent cellular cytotoxicity. Interestingly, G7 mAb induces dramatic levels of granulocyte killing against nucleated K562 targets. These results suggest that G7 mAb recognizes a trigger molecule involved in porcine cellular cytotoxicity.

AB - Monoclonal antibody (mAb) G7 has been developed and appears to recognize a triggering structure on porcine natural killer (NK) cells and granulocytes. G7 mAb binds to approximately 13% of lymphocytes, 70% of monocytes, and >95% of granulocytes. G7 mAb does not react with B cells. G7 mAb immunoprecipitates a heterodispersed molecule of ~40 kDa. Functionally, whole but not F(ab′)2 fragments of G7 mAb enhance NK killing of Fc receptor positive K562, U937, and MOLT-4 targets but not Fc receptor negative CEM, WEHI-164, or YAC-1 targets. Both whole and F(ab′)2 fragments of G7 mAb inhibit lymphocyte-mediated antibody-dependent cellular cytotoxicity. Interestingly, G7 mAb induces dramatic levels of granulocyte killing against nucleated K562 targets. These results suggest that G7 mAb recognizes a trigger molecule involved in porcine cellular cytotoxicity.

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A triggering structure recognized by G7 monoclonal antibody on porcine lymphocytes and granulocytes

Abstract

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