TY - JOUR
T1 - Acquisition, preparation, and functional assessment of human NK cells for adoptive immunotherapy.
AU - Lee, Dean A.
AU - Verneris, Michael R.
AU - Campana, Dario
N1 - Copyright:
MEDLINE® is the source for the citation and abstract of this record.
PY - 2010
Y1 - 2010
N2 - Human natural killer (NK) cells, a subset of peripheral blood lymphocytes that lack a T- or B-cell receptor, play a crucial role in the innate immune response to viruses and malignant cells. NK cells differentiate infected or malignant cells from normal cells by a complex balance between activating and inhibitory receptor-ligand interactions. Unlike T cells, NK cells do not proliferate in vitro in response to simple crosslinking of a single activating receptor. While many methods to study T-cell function and phenotype can also be applied to NK cells, this chapter addresses methods that are unique to the preparation and assessment of human NK cells for immunotherapy.
AB - Human natural killer (NK) cells, a subset of peripheral blood lymphocytes that lack a T- or B-cell receptor, play a crucial role in the innate immune response to viruses and malignant cells. NK cells differentiate infected or malignant cells from normal cells by a complex balance between activating and inhibitory receptor-ligand interactions. Unlike T cells, NK cells do not proliferate in vitro in response to simple crosslinking of a single activating receptor. While many methods to study T-cell function and phenotype can also be applied to NK cells, this chapter addresses methods that are unique to the preparation and assessment of human NK cells for immunotherapy.
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U2 - 10.1007/978-1-60761-786-0_4
DO - 10.1007/978-1-60761-786-0_4
M3 - Article
C2 - 20686960
AN - SCOPUS:78149253287
SN - 1064-3745
VL - 651
SP - 61
EP - 77
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -