Activation of p21WAF1/Cip1 expression by a temperature-sensitive mutant of human p53 does not lead to apoptosis

Tohru Kobayashi; Ugo Consoli; Michael Andreeff; Hiroshi Shiku; Albert B. Deisseroth; Wei Zhang

Activation of p21^WAF1/Cip1 expression by a temperature-sensitive mutant of human p53 does not lead to apoptosis

Tohru Kobayashi, Ugo Consoli, Michael Andreeff, Hiroshi Shiku, Albert B. Deisseroth, Wei Zhang

Research output: Contribution to journal › Article › peer-review

Abstract

The cyclin-dependent kinase inhibitor p21^WAF1/Cip1 (p21) inhibits cellular proliferation and has been implicated in p53-dependent apoptosis. In this study, we examined the regulation of p21 in the K562 erythroleukemia cell line using a human temperature-sensitive mutant of p53, 143Ala. We showed that 143Ala at the permissive temperature (32°C) activated the expression of p21. Stable cell lines expressing 143Ala or other p53 mutants were established. We then examined whether elevation of p21 promotes apoptosis and sensitizes cells to radiation-induced apoptosis. Our results showed that p21 elevation did not promote or sensitize K562 cells to apoptosis.

Original language	English (US)
Pages (from-to)	2311-2316
Number of pages	6
Journal	Oncogene
Volume	11
Issue number	11
State	Published - Dec 7 1995

Keywords

Apoptosis
K562 cells
Temperature-sensitive p53
p21

ASJC Scopus subject areas

Molecular Biology
Genetics
Cancer Research

Cite this

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title = "Activation of p21WAF1/Cip1 expression by a temperature-sensitive mutant of human p53 does not lead to apoptosis",

abstract = "The cyclin-dependent kinase inhibitor p21WAF1/Cip1 (p21) inhibits cellular proliferation and has been implicated in p53-dependent apoptosis. In this study, we examined the regulation of p21 in the K562 erythroleukemia cell line using a human temperature-sensitive mutant of p53, 143Ala. We showed that 143Ala at the permissive temperature (32°C) activated the expression of p21. Stable cell lines expressing 143Ala or other p53 mutants were established. We then examined whether elevation of p21 promotes apoptosis and sensitizes cells to radiation-induced apoptosis. Our results showed that p21 elevation did not promote or sensitize K562 cells to apoptosis.",

keywords = "Apoptosis, K562 cells, Temperature-sensitive p53, p21",

author = "Tohru Kobayashi and Ugo Consoli and Michael Andreeff and Hiroshi Shiku and Deisseroth, {Albert B.} and Wei Zhang",

year = "1995",

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T1 - Activation of p21WAF1/Cip1 expression by a temperature-sensitive mutant of human p53 does not lead to apoptosis

AU - Kobayashi, Tohru

AU - Consoli, Ugo

AU - Andreeff, Michael

AU - Shiku, Hiroshi

AU - Deisseroth, Albert B.

AU - Zhang, Wei

PY - 1995/12/7

Y1 - 1995/12/7

N2 - The cyclin-dependent kinase inhibitor p21WAF1/Cip1 (p21) inhibits cellular proliferation and has been implicated in p53-dependent apoptosis. In this study, we examined the regulation of p21 in the K562 erythroleukemia cell line using a human temperature-sensitive mutant of p53, 143Ala. We showed that 143Ala at the permissive temperature (32°C) activated the expression of p21. Stable cell lines expressing 143Ala or other p53 mutants were established. We then examined whether elevation of p21 promotes apoptosis and sensitizes cells to radiation-induced apoptosis. Our results showed that p21 elevation did not promote or sensitize K562 cells to apoptosis.

AB - The cyclin-dependent kinase inhibitor p21WAF1/Cip1 (p21) inhibits cellular proliferation and has been implicated in p53-dependent apoptosis. In this study, we examined the regulation of p21 in the K562 erythroleukemia cell line using a human temperature-sensitive mutant of p53, 143Ala. We showed that 143Ala at the permissive temperature (32°C) activated the expression of p21. Stable cell lines expressing 143Ala or other p53 mutants were established. We then examined whether elevation of p21 promotes apoptosis and sensitizes cells to radiation-induced apoptosis. Our results showed that p21 elevation did not promote or sensitize K562 cells to apoptosis.

KW - Apoptosis

KW - K562 cells

KW - Temperature-sensitive p53

KW - p21

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ER -

Activation of p21^WAF1/Cip1 expression by a temperature-sensitive mutant of human p53 does not lead to apoptosis

Abstract

Keywords

ASJC Scopus subject areas

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