TY - JOUR
T1 - Active macromolecule uptake by lymph node antigen-presenting cells
T2 - A novel mechanism in determining sentinel lymph node status
AU - Faries, Mark B.
AU - Bedrosian, Isabelle
AU - Reynolds, Carol
AU - Nguyen, Hung Q.
AU - Alavi, Abass
AU - Czerniecki, Brian J.
N1 - Funding Information:
Acknowledgments: M.B.F. and I.B. are supported by NIH Surgical Oncology Training Grant 2-T32-CA096-19-11. B.J.C. is supported by a Career Development Award from the American Cancer Society.
PY - 2000/3
Y1 - 2000/3
N2 - Background: Although sentinel lymph node (SLN) biopsy is a powerful staging tool for patients with melanoma and breast cancer, controversy remains regarding specific aspects of technique. We examined particle uptake by antigen-presenting cells (APCs) to determine if this mechanism is responsible for the differential retention of radioactivity in SLNs relative to nonsentinel lymph nodes (NSLNs). Methods: Mapping was conducted in pigs injected with vital blue dye, fluoroscein isothiocyanate-labeled human serum albumin (FITC-HSA), and one of two 99mtechnetium-labeled tracers, i.e., human serum albumin, a small macromolecule, or unfiltered sulfur colloid, a mixture of small and large particles. Macromolecule uptake by APCs was studied in vitro by using FITC-HSA and measured by fluorescence-activated cell sorting (FACS). SLNs and NSLNs were analyzed by fluorescence microscopy or FACS, with counterstaining for leukocyte cell surface markers. Results: Both radiotracers were effective. Cultured APCs rapidly took up FITC-HSA. Microscopy showed FITC-HSA in the subcapsular sinus of SLNs shortly after injection and subsequent distribution to interfollicular areas. FACS revealed increasing amounts of FITC-HSA in SLNs over time. Cells responsible for uptake were APCs, expressing major histocompatibility (locus) class II. Conclusions: This report establishes active macromolecule uptake as a mechanism that determines SLN status. This mechanism has important implications for performing SLN biopsy.
AB - Background: Although sentinel lymph node (SLN) biopsy is a powerful staging tool for patients with melanoma and breast cancer, controversy remains regarding specific aspects of technique. We examined particle uptake by antigen-presenting cells (APCs) to determine if this mechanism is responsible for the differential retention of radioactivity in SLNs relative to nonsentinel lymph nodes (NSLNs). Methods: Mapping was conducted in pigs injected with vital blue dye, fluoroscein isothiocyanate-labeled human serum albumin (FITC-HSA), and one of two 99mtechnetium-labeled tracers, i.e., human serum albumin, a small macromolecule, or unfiltered sulfur colloid, a mixture of small and large particles. Macromolecule uptake by APCs was studied in vitro by using FITC-HSA and measured by fluorescence-activated cell sorting (FACS). SLNs and NSLNs were analyzed by fluorescence microscopy or FACS, with counterstaining for leukocyte cell surface markers. Results: Both radiotracers were effective. Cultured APCs rapidly took up FITC-HSA. Microscopy showed FITC-HSA in the subcapsular sinus of SLNs shortly after injection and subsequent distribution to interfollicular areas. FACS revealed increasing amounts of FITC-HSA in SLNs over time. Cells responsible for uptake were APCs, expressing major histocompatibility (locus) class II. Conclusions: This report establishes active macromolecule uptake as a mechanism that determines SLN status. This mechanism has important implications for performing SLN biopsy.
KW - - Technetium-sulfur colloid
KW - Antigen uptake
KW - Sentinel lymph node Technetium-human serum albumin
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U2 - 10.1007/s10434-000-0098-6
DO - 10.1007/s10434-000-0098-6
M3 - Article
C2 - 10761787
AN - SCOPUS:0034068201
SN - 1068-9265
VL - 7
SP - 98
EP - 105
JO - Annals of surgical oncology
JF - Annals of surgical oncology
IS - 2
ER -