Abstract
We tested the activity of Novispirin G-10, a novel antimicrobial α-helical octadecapeptide structurally related to cathelicidins and other innate immunity peptides, against Chlamydia trachomatis serovars L2, D, and E and three organisms associated with bacterial vaginosis (BV). The peptide's activity against C. trachomatis was measured in 48-h shell vial assays with McCoy cell targets. Exposure to 100 μg/ml of Novispirin G-10 reduced the infectivity of serovars D and E by 99.4-100% and serovar L2 by 91.7-99.1%. At the same concentration of 100 μg/ml, Novispirin G-10 rapidly killed >99% of Mobiluncus curtisii, Gardnerella vaginalis, and Prevotella bivia, in standard colony-forming unit (CFU) assays. Given its simple structure and relative lack of cytotoxic and hemolytic activity, Novispirin G-10 may be a useful component of microbicide preparations designed to prevent chlamydial infection and/or remediate the abnormal vaginal flora associated with BV.
Original language | English (US) |
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Pages (from-to) | 190-195 |
Number of pages | 6 |
Journal | Experimental and Molecular Pathology |
Volume | 74 |
Issue number | 2 |
DOIs | |
State | Published - Apr 2003 |
Keywords
- Antimicrobial peptides
- Bacterial vaginosis
- Cathelicidin
- Chlamydia trachomatis
- Gardnerella vaginalis
- Mobiluncus curtisii
- Novispirin
- Prevotella bivia
- Protegrin
- Sexually transmitted diseases
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Molecular Biology
- Clinical Biochemistry