Abstract
OBJECTIVE: To evaluate the expression and activity of TNF-related apoptosis-inducing ligand (TRAIL) gene expressed from the hTERT promoter on colon cancer cell line HT-29. METHODS: GFP/TRAIL gene expressed from the hTERT promoter was transfected into HT-29 with adenoviral vectors system, expression and apoptosis inducing ability of GFP/TRAIL protein were determined with fluorescence-activated cell sorting (FACS) method. RESULTS: The expression of GFP gene was 31.4 % and 67.0 % with either hTERT promoter or CMV promoter in DLD1 cells; GFP/TRAIL gene was able to inhibit cell growth (74.2%) and induce apoptosis (25.8%) of HT-29 cells. There was significant difference between Ad/hTERT-gTRAIL and the other two control groups (PBS and Ad/CMV-GFP, P<0.05). CONCLUSION: The GFP/TRAIL gene with hTERT promoter transfected by adenoviral vector was successfully expressed in HT-29 cell, which can both inhibit cell growth and induce apoptosis of colon cancer cell line HT-29.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 45-49 |
| Number of pages | 5 |
| Journal | Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences |
| Volume | 35 |
| Issue number | 1 |
| State | Published - Jan 2006 |
ASJC Scopus subject areas
- General Medicine