TY - JOUR
T1 - Acute lymphoblastic leukemia
T2 - Survey of immunophenotype, French- American-British classification, frequency of myeloid antigen expression, and karyotypic abnormalities in 210 pediatric and adult cases
AU - Khalidi, Hasan S.
AU - Chang, Karen L.
AU - Medeiros, L. Jeffrey
AU - Brynes, Russell K.
AU - Slovak, Marilyn L.
AU - Murata-Collins, Joyce L.
AU - Arber, Daniel A.
PY - 1999
Y1 - 1999
N2 - Immunophenotypic studies are essential to distinguish acute lymphoblastic leukemia (ALL) from minimally differentiated acute myeloid leukemia (AML-M0) and to classify ALL into immunologic subtypes. Frequently, immunophenotyping identifies myeloid antigen expression in ALL, causing a potential diagnostic problem. To evaluate the immunophenotype of ALL, we studied 210 cases of pediatric and adult ALL by flow cytometry and compared the results with the French-American-British (FAB) Cooperative Group classification and the karyotypic findings. Myeloid-associated antigens were expressed in 78 (45.6%) of precursor B-cell ALL cases. Pediatric precursor BALLs had a higher frequency of myeloid antigen expression than did adult cases. All mature B-cell ALL cases were negative for TdT and myeloid antigens. Myeloid antigen expression was less frequent in T-cell ALL cases compared with precursor B-cell ALL cases. Of the 192 cases submitted for cytogenetic analysis, 147 were abnormal. The mast common chromosomal translocation was the Philadelphia chromosome, which was more likely to have L2 blast morphology and a precursor B immunophenotype. Myeloid antigen expression was present in 70.8% of Ph-positive cases (P = .008). Chromosome rearrangements involving 11q23 also showed an increased frequency of myeloid antigen expression. Chromosome translocations involving regions of T-cell receptor genes were present in 24% of T-cell ALL cases. A high percentage of ALL cases, however, had various other cytogenetic abnormalities, many of which involved less well-studied chromosomal regions.
AB - Immunophenotypic studies are essential to distinguish acute lymphoblastic leukemia (ALL) from minimally differentiated acute myeloid leukemia (AML-M0) and to classify ALL into immunologic subtypes. Frequently, immunophenotyping identifies myeloid antigen expression in ALL, causing a potential diagnostic problem. To evaluate the immunophenotype of ALL, we studied 210 cases of pediatric and adult ALL by flow cytometry and compared the results with the French-American-British (FAB) Cooperative Group classification and the karyotypic findings. Myeloid-associated antigens were expressed in 78 (45.6%) of precursor B-cell ALL cases. Pediatric precursor BALLs had a higher frequency of myeloid antigen expression than did adult cases. All mature B-cell ALL cases were negative for TdT and myeloid antigens. Myeloid antigen expression was less frequent in T-cell ALL cases compared with precursor B-cell ALL cases. Of the 192 cases submitted for cytogenetic analysis, 147 were abnormal. The mast common chromosomal translocation was the Philadelphia chromosome, which was more likely to have L2 blast morphology and a precursor B immunophenotype. Myeloid antigen expression was present in 70.8% of Ph-positive cases (P = .008). Chromosome rearrangements involving 11q23 also showed an increased frequency of myeloid antigen expression. Chromosome translocations involving regions of T-cell receptor genes were present in 24% of T-cell ALL cases. A high percentage of ALL cases, however, had various other cytogenetic abnormalities, many of which involved less well-studied chromosomal regions.
KW - Acute lymphoblastic leukemia
KW - Cytogenetics
KW - Flow cytometry
KW - French-American-British Cooperative Group classification
KW - Immunophenotyping
KW - Myeloid antigens
UR - http://www.scopus.com/inward/record.url?scp=0033054730&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033054730&partnerID=8YFLogxK
U2 - 10.1093/ajcp/111.4.467
DO - 10.1093/ajcp/111.4.467
M3 - Article
C2 - 10191766
AN - SCOPUS:0033054730
SN - 0002-9173
VL - 111
SP - 467
EP - 476
JO - American journal of clinical pathology
JF - American journal of clinical pathology
IS - 4
ER -