TY - JOUR
T1 - Adenoviral Vector as a Gene Delivery System into Cultured Rat Neuronal and Glial Cells
AU - Caillaud, Catherine
AU - Akli, Said
AU - Vigne, Emmanuelle
AU - Koulakoff, Annette
AU - Perricaudet, Michel
AU - Poenaru, Livia
AU - Kahn, Axel
AU - Berwald‐Netter, Yoheved
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1993/10
Y1 - 1993/10
N2 - Previous studies have demonstrated that a defective recombinant adenovirus can infect a wide range of postmitotic and slowly proliferating cell types such as hepatocytes, myotubes, pneumocytes and intestinal cells (Stratford‐Perricaudet et al., Hum. Gene Ther., 1, 241–256, 1990; Quantin et al., Proc. Natl. Acad. Sci. USA, 89, 2581–2584, 1992; Jaffe et al., Nature Genetics, 1, 372–378, 1992). We have used a defective recombinant adenovirus, Ad.RSVβgal, containing the Escherichia coliβ‐galactosidase gene targeted to the nucleus under the transcriptional control of the Rous sarcoma virus long terminal repeat promoter (Stratford‐Perricaudet et al., J. Clin. Invest., 90, 626–630, 1992) to infect non‐dividing neural cells in primary culture. We show that 80–100% of neuronal and astroglial cells infected with a viral titre lower than 109 p.f.u./ml express β‐galactosidase for at least 1 month without cell damage. These results demonstrate the potential usefulness of recombinant adenovirus infection for the analysis of brain‐specific gene regulation and for the transfer of genes into neural cells before their transplantation into the brain.
AB - Previous studies have demonstrated that a defective recombinant adenovirus can infect a wide range of postmitotic and slowly proliferating cell types such as hepatocytes, myotubes, pneumocytes and intestinal cells (Stratford‐Perricaudet et al., Hum. Gene Ther., 1, 241–256, 1990; Quantin et al., Proc. Natl. Acad. Sci. USA, 89, 2581–2584, 1992; Jaffe et al., Nature Genetics, 1, 372–378, 1992). We have used a defective recombinant adenovirus, Ad.RSVβgal, containing the Escherichia coliβ‐galactosidase gene targeted to the nucleus under the transcriptional control of the Rous sarcoma virus long terminal repeat promoter (Stratford‐Perricaudet et al., J. Clin. Invest., 90, 626–630, 1992) to infect non‐dividing neural cells in primary culture. We show that 80–100% of neuronal and astroglial cells infected with a viral titre lower than 109 p.f.u./ml express β‐galactosidase for at least 1 month without cell damage. These results demonstrate the potential usefulness of recombinant adenovirus infection for the analysis of brain‐specific gene regulation and for the transfer of genes into neural cells before their transplantation into the brain.
KW - brain transplantation
KW - brain‐specific gene regulation
KW - defective recombinant adenovirus
KW - β‐galactosidase
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U2 - 10.1111/j.1460-9568.1993.tb00914.x
DO - 10.1111/j.1460-9568.1993.tb00914.x
M3 - Article
C2 - 8275231
AN - SCOPUS:0027432367
SN - 0953-816X
VL - 5
SP - 1287
EP - 1291
JO - European Journal of Neuroscience
JF - European Journal of Neuroscience
IS - 10
ER -