TY - JOUR
T1 - Adenovirus-mediated transfer of the p53 gene produces rapid and generalized death of human glioma cells via apoptosis
AU - Gomez-Manzano, Candelaria
AU - Fueyo, Juan
AU - Kyritsis, Athanassios P.
AU - Steck, Peter A.
AU - Roth, Jack A.
AU - McDonnell, Timothy J.
AU - Steck, Kim D.
AU - Levin, Victor A.
AU - Yung, W. K.Alfred
PY - 1996/2/15
Y1 - 1996/2/15
N2 - Wild-type p53 is involved in several aspects of cell cycle control and suppression of transformation, inducing either apoptosis or G1 block in cell cycle progression. Using a recombinant adenovirus containing the wild-type p53 cDNA, the biological effects of the newly expressed wild-type p53 protein were examined in six human glioma cell lines. Three cell lines (U-251 MG, U- 373 MG, and A-172) expressed endogenous mutant p53, and the other three (U- 87 MG, EFC-2, and D54 MG) expressed wild-type p53. The restoration of normal p53-encoded protein in the mutant cell lines induced apoptosis as assessed by morphological studies using nuclear staining, electron microscopy, and flow cytometric assays. In wild-type p53 cell lines, however, the overexpression of wild-type p53 did not result in apoptosis but inhibited cellular proliferation rather drastically and modified the neoplastic phenotype. Differential effects suggest two pathways for glioma oncogenesis and a possible therapeutic strategy.
AB - Wild-type p53 is involved in several aspects of cell cycle control and suppression of transformation, inducing either apoptosis or G1 block in cell cycle progression. Using a recombinant adenovirus containing the wild-type p53 cDNA, the biological effects of the newly expressed wild-type p53 protein were examined in six human glioma cell lines. Three cell lines (U-251 MG, U- 373 MG, and A-172) expressed endogenous mutant p53, and the other three (U- 87 MG, EFC-2, and D54 MG) expressed wild-type p53. The restoration of normal p53-encoded protein in the mutant cell lines induced apoptosis as assessed by morphological studies using nuclear staining, electron microscopy, and flow cytometric assays. In wild-type p53 cell lines, however, the overexpression of wild-type p53 did not result in apoptosis but inhibited cellular proliferation rather drastically and modified the neoplastic phenotype. Differential effects suggest two pathways for glioma oncogenesis and a possible therapeutic strategy.
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M3 - Article
C2 - 8630997
AN - SCOPUS:0030020689
SN - 0008-5472
VL - 56
SP - 694
EP - 699
JO - Cancer Research
JF - Cancer Research
IS - 4
ER -