Adipokine gene expression in adipocytes isolated from different fat depots of coronary artery disease patients

Maxim Yu Sinitsky; Yulia A. Dyleva; Evgenya G. Uchasova; Ekaterina V. Belik; Arseniy E. Yuzhalin; Olga V. Gruzdeva; Vera G. Matveeva; Anastasia V. Ponasenko

doi:10.1080/13813455.2019.1674338

Adipokine gene expression in adipocytes isolated from different fat depots of coronary artery disease patients

Maxim Yu Sinitsky, Yulia A. Dyleva, Evgenya G. Uchasova, Ekaterina V. Belik, Arseniy E. Yuzhalin, Olga V. Gruzdeva, Vera G. Matveeva, Anastasia V. Ponasenko

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

To compare DPP4, LCN2, NAMPT, ITLN1, APLN mRNA levels in adipocytes isolated from the biopsies of subcutaneous, epicardial and perivascular fat obtained from 25 patients with coronary artery disease. Gene expression signature was determined by RT-qPCR with hydrolysis probes. We found DPP4 and APLN mRNA was higher expressed only in adipocytes isolated from epicardial adipose tissue compared to the subcutaneous fat. The ITLN1 gene was overexpressed in epicardial adipose tissue compared to both subcutaneous and perivascular tissues. APLN mRNA expression was positively correlated with total and LDL cholesterol plasma level, and DPP4 mRNA expression–with VLDL cholesterol concentration. Thus, adipocytes isolated from different adipose depots are characterised by differential gene expression of adipokines. Epicardial adipose tissue is of particular interest in the context of its function, molecular and genetic mechanisms of regulation of the cardiovascular system and as a therapeutic target for correction of adipose tissue-induced effects on health.

Original language	English (US)
Pages (from-to)	261-269
Number of pages	9
Journal	Archives of Physiology and Biochemistry
Volume	128
Issue number	1
DOIs	https://doi.org/10.1080/13813455.2019.1674338
State	Published - 2022
Externally published	Yes

Keywords

Adipokines
adipose tissue
coronary artery disease
gene expression
mRNA

ASJC Scopus subject areas

Physiology
Physiology (medical)

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10.1080/13813455.2019.1674338

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title = "Adipokine gene expression in adipocytes isolated from different fat depots of coronary artery disease patients",

abstract = "To compare DPP4, LCN2, NAMPT, ITLN1, APLN mRNA levels in adipocytes isolated from the biopsies of subcutaneous, epicardial and perivascular fat obtained from 25 patients with coronary artery disease. Gene expression signature was determined by RT-qPCR with hydrolysis probes. We found DPP4 and APLN mRNA was higher expressed only in adipocytes isolated from epicardial adipose tissue compared to the subcutaneous fat. The ITLN1 gene was overexpressed in epicardial adipose tissue compared to both subcutaneous and perivascular tissues. APLN mRNA expression was positively correlated with total and LDL cholesterol plasma level, and DPP4 mRNA expression–with VLDL cholesterol concentration. Thus, adipocytes isolated from different adipose depots are characterised by differential gene expression of adipokines. Epicardial adipose tissue is of particular interest in the context of its function, molecular and genetic mechanisms of regulation of the cardiovascular system and as a therapeutic target for correction of adipose tissue-induced effects on health.",

keywords = "Adipokines, adipose tissue, coronary artery disease, gene expression, mRNA",

author = "Sinitsky, {Maxim Yu} and Dyleva, {Yulia A.} and Uchasova, {Evgenya G.} and Belik, {Ekaterina V.} and Yuzhalin, {Arseniy E.} and Gruzdeva, {Olga V.} and Matveeva, {Vera G.} and Ponasenko, {Anastasia V.}",

note = "Publisher Copyright: {\textcopyright} 2019 Informa UK Limited, trading as Taylor & Francis Group.",

year = "2022",

doi = "10.1080/13813455.2019.1674338",

language = "English (US)",

volume = "128",

pages = "261--269",

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T1 - Adipokine gene expression in adipocytes isolated from different fat depots of coronary artery disease patients

AU - Sinitsky, Maxim Yu

AU - Dyleva, Yulia A.

AU - Uchasova, Evgenya G.

AU - Belik, Ekaterina V.

AU - Yuzhalin, Arseniy E.

AU - Gruzdeva, Olga V.

AU - Matveeva, Vera G.

AU - Ponasenko, Anastasia V.

PY - 2022

Y1 - 2022

N2 - To compare DPP4, LCN2, NAMPT, ITLN1, APLN mRNA levels in adipocytes isolated from the biopsies of subcutaneous, epicardial and perivascular fat obtained from 25 patients with coronary artery disease. Gene expression signature was determined by RT-qPCR with hydrolysis probes. We found DPP4 and APLN mRNA was higher expressed only in adipocytes isolated from epicardial adipose tissue compared to the subcutaneous fat. The ITLN1 gene was overexpressed in epicardial adipose tissue compared to both subcutaneous and perivascular tissues. APLN mRNA expression was positively correlated with total and LDL cholesterol plasma level, and DPP4 mRNA expression–with VLDL cholesterol concentration. Thus, adipocytes isolated from different adipose depots are characterised by differential gene expression of adipokines. Epicardial adipose tissue is of particular interest in the context of its function, molecular and genetic mechanisms of regulation of the cardiovascular system and as a therapeutic target for correction of adipose tissue-induced effects on health.

AB - To compare DPP4, LCN2, NAMPT, ITLN1, APLN mRNA levels in adipocytes isolated from the biopsies of subcutaneous, epicardial and perivascular fat obtained from 25 patients with coronary artery disease. Gene expression signature was determined by RT-qPCR with hydrolysis probes. We found DPP4 and APLN mRNA was higher expressed only in adipocytes isolated from epicardial adipose tissue compared to the subcutaneous fat. The ITLN1 gene was overexpressed in epicardial adipose tissue compared to both subcutaneous and perivascular tissues. APLN mRNA expression was positively correlated with total and LDL cholesterol plasma level, and DPP4 mRNA expression–with VLDL cholesterol concentration. Thus, adipocytes isolated from different adipose depots are characterised by differential gene expression of adipokines. Epicardial adipose tissue is of particular interest in the context of its function, molecular and genetic mechanisms of regulation of the cardiovascular system and as a therapeutic target for correction of adipose tissue-induced effects on health.

KW - Adipokines

KW - adipose tissue

KW - coronary artery disease

KW - gene expression

KW - mRNA

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IS - 1

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Adipokine gene expression in adipocytes isolated from different fat depots of coronary artery disease patients

Abstract

Keywords

ASJC Scopus subject areas

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