TY - JOUR
T1 - Aged marrow macrophages expand platelet-biased hematopoietic stem cells via interleukin-1B
AU - Frisch, Benjamin J.
AU - Hoffman, Corey M.
AU - Latchney, Sarah E.
AU - LaMere, Mark W.
AU - Myers, Jason
AU - Ashton, John
AU - Li, Allison J.
AU - Saunders, Jerry
AU - Palis, James
AU - Perkins, Archibald S.
AU - McCabe, Amanda
AU - Smith, Julianne N.P.
AU - McGrath, Kathleen E.
AU - Rivera-Escalera, Fatima
AU - McDavid, Andrew
AU - Liesveld, Jane L.
AU - Korshunov, Vyacheslav A.
AU - Elliott, Michael R.
AU - MacNamara, Katherine C.
AU - Becker, Michael W.
AU - Calvi, Laura M.
N1 - Publisher Copyright:
© 2019 American Society for Clinical Investigation.
PY - 2019
Y1 - 2019
N2 - The bone marrow microenvironment contributes to the regulation of hematopoietic stem cell (HSC) function, though its role in age-associated lineage skewing is poorly understood. Here we show that dysfunction of aged marrow macrophages (Mφs) directs HSC platelet bias. Mφs from the marrow of aged mice and humans exhibited an activated phenotype, with increased expression of inflammatory signals. Aged marrow Mφs also displayed decreased phagocytic function. Senescent neutrophils, typically cleared by marrow Mφs, were markedly increased in aged mice, consistent with functional defects in Mφ phagocytosis and efferocytosis. In aged mice, interleukin-1B (IL-1B) was elevated in the bone marrow, and caspase-1 activity, which can process pro-IL-1B, was increased in marrow Mφs and neutrophils. Mechanistically, IL-1B signaling was necessary and sufficient to induce a platelet bias in HSCs. In young mice, depletion of phagocytic cell populations or loss of the efferocytic receptor Axl expanded platelet-biased HSCs. Our data support a model wherein increased inflammatory signals and decreased phagocytic function of aged marrow Mφs induce the acquisition of platelet bias in aged HSCs. This work highlights the instructive role of Mφs and IL-1B in the age-associated lineage skewing of HSCs, and reveals the therapeutic potential of their manipulation as antigeronic targets.
AB - The bone marrow microenvironment contributes to the regulation of hematopoietic stem cell (HSC) function, though its role in age-associated lineage skewing is poorly understood. Here we show that dysfunction of aged marrow macrophages (Mφs) directs HSC platelet bias. Mφs from the marrow of aged mice and humans exhibited an activated phenotype, with increased expression of inflammatory signals. Aged marrow Mφs also displayed decreased phagocytic function. Senescent neutrophils, typically cleared by marrow Mφs, were markedly increased in aged mice, consistent with functional defects in Mφ phagocytosis and efferocytosis. In aged mice, interleukin-1B (IL-1B) was elevated in the bone marrow, and caspase-1 activity, which can process pro-IL-1B, was increased in marrow Mφs and neutrophils. Mechanistically, IL-1B signaling was necessary and sufficient to induce a platelet bias in HSCs. In young mice, depletion of phagocytic cell populations or loss of the efferocytic receptor Axl expanded platelet-biased HSCs. Our data support a model wherein increased inflammatory signals and decreased phagocytic function of aged marrow Mφs induce the acquisition of platelet bias in aged HSCs. This work highlights the instructive role of Mφs and IL-1B in the age-associated lineage skewing of HSCs, and reveals the therapeutic potential of their manipulation as antigeronic targets.
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U2 - 10.1172/jci.insight.124213
DO - 10.1172/jci.insight.124213
M3 - Article
C2 - 30998506
AN - SCOPUS:85070658611
SN - 2379-3708
VL - 4
JO - JCI Insight
JF - JCI Insight
IS - 10
M1 - e124213
ER -