TY - JOUR
T1 - Alcohol-induced oxidative stress in rat liver microsomes
T2 - Protective effect of Emblica officinalis
AU - Reddy, Vaddi Damodara
AU - Padmavathi, Pannuru
AU - Hymavathi, Reddyvari
AU - Maturu, Paramahamsa
AU - Varadacharyulu, N. Ch
N1 - Funding Information:
The authors thank the Director and staff of the Center for Cellular and Molecular Biology, Hyderabad, India for providing facilities to carry out fluidity studies. This study was supported in part by the University Grants Commission, New Delhi, India (Grant No. F.No.40-203/2011 SR ).
PY - 2014/6
Y1 - 2014/6
N2 - The protective effect of Emblica officinalis fruit extract (EFE) against alcohol-induced oxidative damage in liver microsomes was investigated in rats. EFE (250mg/kg b.wt/day) and alcohol (5g/kg b.wt/day, 20%, w/v) were administered orally to animals for 60 days. Alcohol administration significantly increased lipid peroxidation, protein carbonyls with decreased sulfhydryl groups in microsomes, which were significantly restored to normal levels in EFE and alcohol co-administered rats. Alcohol administration also markedly decreased the levels of reduced glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) in the liver microsomes, which were prevented with EFE administration. Further, alcohol administration significantly increased the activities of cytochrome P-450, Na+/K+ and Mg2+ ATPases and also membrane fluidity. But, administration of EFE along with alcohol restored the all above enzyme activities and membrane fluidity to normal level. Thus, EFE showed protective effects against alcohol-induced oxidative damage by possibly reducing the rate of lipid peroxidation and restoring the various membrane bound and antioxidant enzyme activities to normal levels, and also by protecting the membrane integrity in rat liver microsomes. In conclusion, the polyphenolic compounds including flavonoid and tannoid compounds present in EFE might be playing a major role against alcohol-induced oxidative stress in rats.
AB - The protective effect of Emblica officinalis fruit extract (EFE) against alcohol-induced oxidative damage in liver microsomes was investigated in rats. EFE (250mg/kg b.wt/day) and alcohol (5g/kg b.wt/day, 20%, w/v) were administered orally to animals for 60 days. Alcohol administration significantly increased lipid peroxidation, protein carbonyls with decreased sulfhydryl groups in microsomes, which were significantly restored to normal levels in EFE and alcohol co-administered rats. Alcohol administration also markedly decreased the levels of reduced glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) in the liver microsomes, which were prevented with EFE administration. Further, alcohol administration significantly increased the activities of cytochrome P-450, Na+/K+ and Mg2+ ATPases and also membrane fluidity. But, administration of EFE along with alcohol restored the all above enzyme activities and membrane fluidity to normal level. Thus, EFE showed protective effects against alcohol-induced oxidative damage by possibly reducing the rate of lipid peroxidation and restoring the various membrane bound and antioxidant enzyme activities to normal levels, and also by protecting the membrane integrity in rat liver microsomes. In conclusion, the polyphenolic compounds including flavonoid and tannoid compounds present in EFE might be playing a major role against alcohol-induced oxidative stress in rats.
KW - Alcohol
KW - CYP-450
KW - Emblica officinalis
KW - Microsomes
KW - Oxidative stress
UR - http://www.scopus.com/inward/record.url?scp=84903156558&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84903156558&partnerID=8YFLogxK
U2 - 10.1016/j.pathophys.2013.12.001
DO - 10.1016/j.pathophys.2013.12.001
M3 - Article
C2 - 24393670
AN - SCOPUS:84903156558
SN - 0928-4680
VL - 21
SP - 153
EP - 159
JO - Pathophysiology
JF - Pathophysiology
IS - 2
ER -