TY - JOUR
T1 - Alkaline phosphatase, glutathione-S-transferase-P, and cofilin-1 distinguish multipotent mesenchymal stromal cell lines derived from the bone marrow versus peripheral blood
AU - Conrad, Claudius
AU - Zeindl-Eberhart, Evelyn
AU - Moosmann, Sabine
AU - Nelson, Peter J.
AU - Bruns, Christiane J.
AU - Huss, Ralf
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2008/2/1
Y1 - 2008/2/1
N2 - Multipotent mesenchymal stromal cells (MSCs) can be isolated from bone marrow or peripheral blood. To identify phenotypical and functional differences between MSCs derived from these sources, the human bone marrow-derived, fibroblast-like cell line L87/4 was compared with the peripheral blood-derived, fibroblast-like cell line V54/2. Both cell lines expressed similar levels of SH3+, CD45-, CD68-, CD133-, and HLA-DR-. The bone marrow-derived cells expressed higher surface levels of CD105, CD10, and CD117 and preferentially expressed alkaline phosphatase, glutathione S-transferase P, and cofilin-1. The peripheral blood-derived line showed a higher number of CD34+/CD105+ double-positive and side population (SP) cells. The results demonstrate the more multipotent, yet quiescent, stromal phenotype of bone marrow MSCs, whereas MSCs isolated from the circulation display more hematopoietic-lineage characteristics. Importantly, potential marker genes that distinguish the two stages of MSCs are defined.
AB - Multipotent mesenchymal stromal cells (MSCs) can be isolated from bone marrow or peripheral blood. To identify phenotypical and functional differences between MSCs derived from these sources, the human bone marrow-derived, fibroblast-like cell line L87/4 was compared with the peripheral blood-derived, fibroblast-like cell line V54/2. Both cell lines expressed similar levels of SH3+, CD45-, CD68-, CD133-, and HLA-DR-. The bone marrow-derived cells expressed higher surface levels of CD105, CD10, and CD117 and preferentially expressed alkaline phosphatase, glutathione S-transferase P, and cofilin-1. The peripheral blood-derived line showed a higher number of CD34+/CD105+ double-positive and side population (SP) cells. The results demonstrate the more multipotent, yet quiescent, stromal phenotype of bone marrow MSCs, whereas MSCs isolated from the circulation display more hematopoietic-lineage characteristics. Importantly, potential marker genes that distinguish the two stages of MSCs are defined.
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U2 - 10.1089/scd.2007.0159
DO - 10.1089/scd.2007.0159
M3 - Article
C2 - 18237266
AN - SCOPUS:39449133645
SN - 1547-3287
VL - 17
SP - 23
EP - 27
JO - Stem Cells and Development
JF - Stem Cells and Development
IS - 1
ER -