Allelic Loss on Chromosome 17 in Ductal Carcinoma in Situ of the Breast

Diane M. Radford; Keri Fair; Alastair M. Thompson; Jon H. Ritter; Matthew Holt; Todd Steinbrueck; Mairi Wallace; Samuel A. Wells; Helen R. Donis-Keller

Allelic Loss on Chromosome 17 in Ductal Carcinoma in Situ of the Breast

Diane M. Radford, Keri Fair, Alastair M. Thompson, Jon H. Ritter, Matthew Holt, Todd Steinbrueck, Mairi Wallace, Samuel A. Wells, Helen R. Donis-Keller

Surgical Oncology

Research output: Contribution to journal › Article › peer-review

76 Scopus citations

Abstract

Multiple tumor suppressor genes are implicated in the oncogenesis and progression of invasive carcinoma of the breast To investigate the chronology of genetic changes we studied loss of heterozygosity on chromosome 17 in ductal carcinoma in situ, a preinvasive breast cancer. A microdissection technique was used to separate tumor from normal stromal cells prior to DNA extraction and loss of heterozygosity was assayed mainly using simple sequence repeat polymorphism markers and the polymerase chain reaction. Loss of heterozygosity on 17p was observed in 8 of 28 tumors (29%) when compared with normal control DNA, whereas no loss was seen on 17q, suggesting that at least one locus on 17p is involved early in the development of breast cancer.

Original language	English (US)
Pages (from-to)	2947-2949
Number of pages	3
Journal	Cancer Research
Volume	53
Issue number	13
State	Published - Aug 1993

ASJC Scopus subject areas

Oncology
Cancer Research

Cite this

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title = "Allelic Loss on Chromosome 17 in Ductal Carcinoma in Situ of the Breast",

abstract = "Multiple tumor suppressor genes are implicated in the oncogenesis and progression of invasive carcinoma of the breast To investigate the chronology of genetic changes we studied loss of heterozygosity on chromosome 17 in ductal carcinoma in situ, a preinvasive breast cancer. A microdissection technique was used to separate tumor from normal stromal cells prior to DNA extraction and loss of heterozygosity was assayed mainly using simple sequence repeat polymorphism markers and the polymerase chain reaction. Loss of heterozygosity on 17p was observed in 8 of 28 tumors (29%) when compared with normal control DNA, whereas no loss was seen on 17q, suggesting that at least one locus on 17p is involved early in the development of breast cancer.",

author = "Radford, {Diane M.} and Keri Fair and Thompson, {Alastair M.} and Ritter, {Jon H.} and Matthew Holt and Todd Steinbrueck and Mairi Wallace and Wells, {Samuel A.} and Donis-Keller, {Helen R.}",

year = "1993",

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journal = "Cancer Research",

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T1 - Allelic Loss on Chromosome 17 in Ductal Carcinoma in Situ of the Breast

AU - Radford, Diane M.

AU - Fair, Keri

AU - Thompson, Alastair M.

AU - Ritter, Jon H.

AU - Holt, Matthew

AU - Steinbrueck, Todd

AU - Wallace, Mairi

AU - Wells, Samuel A.

AU - Donis-Keller, Helen R.

PY - 1993/8

Y1 - 1993/8

N2 - Multiple tumor suppressor genes are implicated in the oncogenesis and progression of invasive carcinoma of the breast To investigate the chronology of genetic changes we studied loss of heterozygosity on chromosome 17 in ductal carcinoma in situ, a preinvasive breast cancer. A microdissection technique was used to separate tumor from normal stromal cells prior to DNA extraction and loss of heterozygosity was assayed mainly using simple sequence repeat polymorphism markers and the polymerase chain reaction. Loss of heterozygosity on 17p was observed in 8 of 28 tumors (29%) when compared with normal control DNA, whereas no loss was seen on 17q, suggesting that at least one locus on 17p is involved early in the development of breast cancer.

AB - Multiple tumor suppressor genes are implicated in the oncogenesis and progression of invasive carcinoma of the breast To investigate the chronology of genetic changes we studied loss of heterozygosity on chromosome 17 in ductal carcinoma in situ, a preinvasive breast cancer. A microdissection technique was used to separate tumor from normal stromal cells prior to DNA extraction and loss of heterozygosity was assayed mainly using simple sequence repeat polymorphism markers and the polymerase chain reaction. Loss of heterozygosity on 17p was observed in 8 of 28 tumors (29%) when compared with normal control DNA, whereas no loss was seen on 17q, suggesting that at least one locus on 17p is involved early in the development of breast cancer.

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AN - SCOPUS:0027170959

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JF - Cancer Research

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ER -

Allelic Loss on Chromosome 17 in Ductal Carcinoma in Situ of the Breast

Abstract

ASJC Scopus subject areas

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