TY - JOUR
T1 - Alteration of cytokine profiles in mice exposed to chronic low-dose ionizing radiation
AU - Shin, Suk Chul
AU - Lee, Kyung Mi
AU - Kang, Yu Mi
AU - Kim, Kwanghee
AU - Kim, Cha Soon
AU - Yang, Kwang Hee
AU - Jin, Young Woo
AU - Kim, Chong Soon
AU - Kim, Hee Sun
N1 - Funding Information:
We thank Miss Hee-won Jang and Miss Seung-yeon Song for helpful experiments. This work was supported by Grant No. E09NS02 from the Korea Hydro & Nuclear Power Co., Ltd. K.-M. Lee is supported by a Grant from KICOS ( K20704000007-09A0500-00710 ) and the National Nuclear R&D program (Grant BAERI). K. Kim is supported by K20601000002-09E0100-00210 .
PY - 2010/7
Y1 - 2010/7
N2 - While a high-dose of ionizing radiation is generally harmful and causes damage to living organisms, a low-dose of radiation has been shown to be beneficial in a variety of animal models. To understand the basis for the effect of low-dose radiation in vivo, we examined the cellular and immunological changes evoked in mice exposed to low-dose radiation at very low (0.7mGy/h) and low (3.95mGy/h) dose rate for the total dose of 0.2 and 2Gy, respectively. Mice exposed to low-dose radiation, either at very low- or low-dose rate, demonstrated normal range of body weight and complete blood counts. Likewise, the number and percentage of peripheral lymphocyte populations, CD4+ T, CD8+ T, B, or NK cells, stayed unchanged following irradiation. Nonetheless, the sera from these mice exhibited elevated levels of IL-3, IL-4, leptin, MCP-1, MCP-5, MIP-1α, thrombopoietin, and VEGF along with slight reduction of IL-12p70, IL-13, IL-17, and IFN-γ. This pattern of cytokine release suggests the stimulation of innate immunity facilitating myeloid differentiation and activation while suppressing pro-inflammatory responses and promoting differentiation of naïve T cells into T-helper 2, not T-helper 1, types. Collectively, our data highlight the subtle changes of cytokine milieu by chronic low-dose γ-radiation, which may be associated with the functional benefits observed in various experimental models.
AB - While a high-dose of ionizing radiation is generally harmful and causes damage to living organisms, a low-dose of radiation has been shown to be beneficial in a variety of animal models. To understand the basis for the effect of low-dose radiation in vivo, we examined the cellular and immunological changes evoked in mice exposed to low-dose radiation at very low (0.7mGy/h) and low (3.95mGy/h) dose rate for the total dose of 0.2 and 2Gy, respectively. Mice exposed to low-dose radiation, either at very low- or low-dose rate, demonstrated normal range of body weight and complete blood counts. Likewise, the number and percentage of peripheral lymphocyte populations, CD4+ T, CD8+ T, B, or NK cells, stayed unchanged following irradiation. Nonetheless, the sera from these mice exhibited elevated levels of IL-3, IL-4, leptin, MCP-1, MCP-5, MIP-1α, thrombopoietin, and VEGF along with slight reduction of IL-12p70, IL-13, IL-17, and IFN-γ. This pattern of cytokine release suggests the stimulation of innate immunity facilitating myeloid differentiation and activation while suppressing pro-inflammatory responses and promoting differentiation of naïve T cells into T-helper 2, not T-helper 1, types. Collectively, our data highlight the subtle changes of cytokine milieu by chronic low-dose γ-radiation, which may be associated with the functional benefits observed in various experimental models.
KW - Adaptive response
KW - Cytokine
KW - Hormesis
KW - Low-dose radiation
KW - Lymphocytes
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U2 - 10.1016/j.bbrc.2010.05.121
DO - 10.1016/j.bbrc.2010.05.121
M3 - Article
C2 - 20513358
AN - SCOPUS:77954383141
SN - 0006-291X
VL - 397
SP - 644
EP - 649
JO - Biochemical and biophysical research communications
JF - Biochemical and biophysical research communications
IS - 4
ER -