Abstract
RNA transcripts derived from the calcitonin (CT)/calcitonin gene-related peptide (CGRP) gene are differentially processed in a tissue-specific fashion to produce two unique mRNAs. This RNA processing decision is deregulated in malignant thyroid C-cells. To examine this mechanism of RNA processing, CT/CGRP minigene constructs were transfected into the human medullary thyroid carcinoma TT cell line. RNA derived from the normal CT/CGRP construct paralleled the endogeneous pathway to produce both CT and CGRP mRNAs. Mutation analysis and RNA/protein crosslinking were performed in order to clarify trans-acting factor interactions. The data suggest that CGRP production in TT cells results from the coexpression of facilitative and inhibitory factors.
Original language | English (US) |
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Pages (from-to) | 993-998 |
Number of pages | 6 |
Journal | Biochemical and biophysical research communications |
Volume | 200 |
Issue number | 2 |
DOIs | |
State | Published - Apr 30 1994 |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology