Alternative splicing of the human MUC2 gene

Lawrence R. Sternberg; James C. Byrd; Gunnar C. Hansson; Kai Feng Liu; Robert S. Bresalier

doi:10.1016/j.abb.2003.10.002

Alternative splicing of the human MUC2 gene

Lawrence R. Sternberg, James C. Byrd, Gunnar C. Hansson, Kai Feng Liu, Robert S. Bresalier

Gastroenterology Hepatology & Nutrition

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Human colon cancers differ in amounts of MUC2 mucin synthesized. However, it is unclear whether MUC2 encodes a single protein. When clones of human colon cancer cells were assayed with antibodies against the TR2 mucin repeat or non-TR2 epitopes, differences in relative expression of MUC2 proteins suggested multiple immunoreactive forms. RT-PCR analysis detected the established 15kbp MUC2 cDNA and a novel form (designated MUC2.1) lacking the MUC2 TR2 repeat. Sequencing of cDNA and genomic DNA indicated that MUC2.1 results from an alternate splice donor. RT-PCR with splice-junction spanning primers confirmed the expression of MUC2.1 mRNA. Anti-MUC2.1 antibody stained colon cancer cells and normal colon in a pattern different from TR2-specific antibody. The presence of MUC2.1 mucin may help us to explain previous conflicting reports that have attempted to correlate the relative abundance of MUC2 protein and/or mRNA with the biological behavior of colon cancer cells.

Original language	English (US)
Pages (from-to)	21-33
Number of pages	13
Journal	Archives of Biochemistry and Biophysics
Volume	421
Issue number	1
DOIs	https://doi.org/10.1016/j.abb.2003.10.002
State	Published - Jan 1 2004

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology

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title = "Alternative splicing of the human MUC2 gene",

abstract = "Human colon cancers differ in amounts of MUC2 mucin synthesized. However, it is unclear whether MUC2 encodes a single protein. When clones of human colon cancer cells were assayed with antibodies against the TR2 mucin repeat or non-TR2 epitopes, differences in relative expression of MUC2 proteins suggested multiple immunoreactive forms. RT-PCR analysis detected the established 15kbp MUC2 cDNA and a novel form (designated MUC2.1) lacking the MUC2 TR2 repeat. Sequencing of cDNA and genomic DNA indicated that MUC2.1 results from an alternate splice donor. RT-PCR with splice-junction spanning primers confirmed the expression of MUC2.1 mRNA. Anti-MUC2.1 antibody stained colon cancer cells and normal colon in a pattern different from TR2-specific antibody. The presence of MUC2.1 mucin may help us to explain previous conflicting reports that have attempted to correlate the relative abundance of MUC2 protein and/or mRNA with the biological behavior of colon cancer cells.",

author = "Sternberg, {Lawrence R.} and Byrd, {James C.} and Hansson, {Gunnar C.} and Liu, {Kai Feng} and Bresalier, {Robert S.}",

note = "Funding Information: This work was supported by National Cancer Institute Grant R01CA69480. The authors acknowledge Neil W. Toribara for providing unpublished MUC2 DNA sequences and thank Chris Yunker for excellent technical assistance. ",

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AU - Sternberg, Lawrence R.

AU - Byrd, James C.

AU - Hansson, Gunnar C.

AU - Liu, Kai Feng

AU - Bresalier, Robert S.

N1 - Funding Information: This work was supported by National Cancer Institute Grant R01CA69480. The authors acknowledge Neil W. Toribara for providing unpublished MUC2 DNA sequences and thank Chris Yunker for excellent technical assistance.

PY - 2004/1/1

Y1 - 2004/1/1

N2 - Human colon cancers differ in amounts of MUC2 mucin synthesized. However, it is unclear whether MUC2 encodes a single protein. When clones of human colon cancer cells were assayed with antibodies against the TR2 mucin repeat or non-TR2 epitopes, differences in relative expression of MUC2 proteins suggested multiple immunoreactive forms. RT-PCR analysis detected the established 15kbp MUC2 cDNA and a novel form (designated MUC2.1) lacking the MUC2 TR2 repeat. Sequencing of cDNA and genomic DNA indicated that MUC2.1 results from an alternate splice donor. RT-PCR with splice-junction spanning primers confirmed the expression of MUC2.1 mRNA. Anti-MUC2.1 antibody stained colon cancer cells and normal colon in a pattern different from TR2-specific antibody. The presence of MUC2.1 mucin may help us to explain previous conflicting reports that have attempted to correlate the relative abundance of MUC2 protein and/or mRNA with the biological behavior of colon cancer cells.

AB - Human colon cancers differ in amounts of MUC2 mucin synthesized. However, it is unclear whether MUC2 encodes a single protein. When clones of human colon cancer cells were assayed with antibodies against the TR2 mucin repeat or non-TR2 epitopes, differences in relative expression of MUC2 proteins suggested multiple immunoreactive forms. RT-PCR analysis detected the established 15kbp MUC2 cDNA and a novel form (designated MUC2.1) lacking the MUC2 TR2 repeat. Sequencing of cDNA and genomic DNA indicated that MUC2.1 results from an alternate splice donor. RT-PCR with splice-junction spanning primers confirmed the expression of MUC2.1 mRNA. Anti-MUC2.1 antibody stained colon cancer cells and normal colon in a pattern different from TR2-specific antibody. The presence of MUC2.1 mucin may help us to explain previous conflicting reports that have attempted to correlate the relative abundance of MUC2 protein and/or mRNA with the biological behavior of colon cancer cells.

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Alternative splicing of the human MUC2 gene

Abstract

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