Alveolar Macrophage Secretory Products Effect Type 2 Pneumocytes Undergoing Hypoxia-Reoxygenation

Anton S. McCourtie, Alexander S. Farivar, Steven M. Woolley, Heather E. Merry, Patrick S. Wolf, Brendan Mackinnon-Patterson, John C. Keech, Elizabeth FitzSullivan, Michael S. Mulligan

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background: Activation of the alveolar macrophage is centrally important to the development of lung ischemia reperfusion injury. Alveolar macrophages and type 2 pneumocytes secrete a variety of proinflammatory mediators in response to oxidative stress. The manner in which they interact and how the macrophage may influence pneumocyte responses in lung ischemia reperfusion injury is unknown. Utilizing an in vitro model of hypoxia and reoxygenation, we sought to determine if the proinflammatory response of type 2 pneumocytes to oxidative stress would be amplified by alveolar macrophage secretory products. Methods: Cultured pneumocytes were exposed to control media or media from cultured macrophages exposed to hypoxia and reoxygenation. Pneumocytes were subsequently subjected to hypoxia and reoxygenation and assessed for both nuclear translocation of nuclear factor kappa B and inflammatory cytokine and chemokine secretion. To examine for any reciprocal interactions, we reversed the experiment, exposing macrophages to conditioned pneumocyte media. Results: In the presence of media from stimulated macrophages, production of proinflammatory mediators by type 2 pneumocytes was dramatically enhanced. In contrast, exposure of the macrophage to conditioned pneumocyte media had an inhibitory effect on macrophage responses subsequently exposed to hypoxia and reoxygenation. Conclusions: The alveolar macrophage drives the development of lung reperfusion injury in part through amplification of the inflammatory response of type 2 pneumocytes subjected to hypoxia and reoxygenation.

Original languageEnglish (US)
Pages (from-to)1774-1779
Number of pages6
JournalAnnals of Thoracic Surgery
Volume86
Issue number6
DOIs
StatePublished - Dec 1 2008

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Alveolar Epithelial Cells
Alveolar Macrophages
Macrophages
Reperfusion Injury
Conditioned Culture Medium
Oxidative Stress
Hypoxia
Lung
NF-kappa B
Lung Injury
Chemokines
Cytokines

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Surgery
  • Pulmonary and Respiratory Medicine

Cite this

McCourtie, A. S., Farivar, A. S., Woolley, S. M., Merry, H. E., Wolf, P. S., Mackinnon-Patterson, B., ... Mulligan, M. S. (2008). Alveolar Macrophage Secretory Products Effect Type 2 Pneumocytes Undergoing Hypoxia-Reoxygenation. Annals of Thoracic Surgery, 86(6), 1774-1779. https://doi.org/10.1016/j.athoracsur.2008.07.071

Alveolar Macrophage Secretory Products Effect Type 2 Pneumocytes Undergoing Hypoxia-Reoxygenation. / McCourtie, Anton S.; Farivar, Alexander S.; Woolley, Steven M.; Merry, Heather E.; Wolf, Patrick S.; Mackinnon-Patterson, Brendan; Keech, John C.; FitzSullivan, Elizabeth; Mulligan, Michael S.

In: Annals of Thoracic Surgery, Vol. 86, No. 6, 01.12.2008, p. 1774-1779.

Research output: Contribution to journalArticle

McCourtie, AS, Farivar, AS, Woolley, SM, Merry, HE, Wolf, PS, Mackinnon-Patterson, B, Keech, JC, FitzSullivan, E & Mulligan, MS 2008, 'Alveolar Macrophage Secretory Products Effect Type 2 Pneumocytes Undergoing Hypoxia-Reoxygenation', Annals of Thoracic Surgery, vol. 86, no. 6, pp. 1774-1779. https://doi.org/10.1016/j.athoracsur.2008.07.071
McCourtie AS, Farivar AS, Woolley SM, Merry HE, Wolf PS, Mackinnon-Patterson B et al. Alveolar Macrophage Secretory Products Effect Type 2 Pneumocytes Undergoing Hypoxia-Reoxygenation. Annals of Thoracic Surgery. 2008 Dec 1;86(6):1774-1779. https://doi.org/10.1016/j.athoracsur.2008.07.071
McCourtie, Anton S. ; Farivar, Alexander S. ; Woolley, Steven M. ; Merry, Heather E. ; Wolf, Patrick S. ; Mackinnon-Patterson, Brendan ; Keech, John C. ; FitzSullivan, Elizabeth ; Mulligan, Michael S. / Alveolar Macrophage Secretory Products Effect Type 2 Pneumocytes Undergoing Hypoxia-Reoxygenation. In: Annals of Thoracic Surgery. 2008 ; Vol. 86, No. 6. pp. 1774-1779.
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AU - Wolf, Patrick S.

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