TY - JOUR
T1 - Amyloid precursor-like protein 2 (APLP2) affects the actin cytoskeleton and increases pancreatic cancer growth and metastasis
AU - Pandey, Poomy
AU - Rachagani, Satyanarayana
AU - Das, Srustidhar
AU - Seshacharyulu, Parthasarathy
AU - Sheinin, Yuri
AU - Naslavsky, Naava
AU - Pan, Zenggang
AU - Smith, Brittney L.
AU - Peters, Haley L.
AU - Radhakrishnan, Prakash
AU - McKenna, Nicole R.
AU - Panapakkam Giridharan, Sai Srinivas
AU - Haridas, Dhanya
AU - Kaur, Sukhwinder
AU - Hollingsworth, Michael A.
AU - MacDonald, Richard G.
AU - Meza, Jane L.
AU - Caplan, Steve
AU - Batra, Surinder K.
AU - Solheim, Joyce C.
PY - 2015
Y1 - 2015
N2 - Amyloid precursor-like protein 2 (APLP2) is aberrantly expressed in pancreatic cancer. Here we showed that APLP2 is increased in pancreatic cancer metastases, particularly in metastatic lesions found in the diaphragm and intestine. Examination of matched human primary tumor-liver metastasis pairs showed that 38.1% of the patients had positive APLP2 expression in both the primary tumor and the corresponding liver metastasis. Stable knock-down of APLP2 expression (with inducible shRNA) in pancreatic cancer cells reduced the ability of these cells to migrate and invade. Loss of APLP2 decreased cortical actin and increased intracellular actin filaments in pancreatic cancer cells. Down-regulation of APLP2 decreased the weight and metastasis of orthotopically transplanted pancreatic tumors in nude mice.
AB - Amyloid precursor-like protein 2 (APLP2) is aberrantly expressed in pancreatic cancer. Here we showed that APLP2 is increased in pancreatic cancer metastases, particularly in metastatic lesions found in the diaphragm and intestine. Examination of matched human primary tumor-liver metastasis pairs showed that 38.1% of the patients had positive APLP2 expression in both the primary tumor and the corresponding liver metastasis. Stable knock-down of APLP2 expression (with inducible shRNA) in pancreatic cancer cells reduced the ability of these cells to migrate and invade. Loss of APLP2 decreased cortical actin and increased intracellular actin filaments in pancreatic cancer cells. Down-regulation of APLP2 decreased the weight and metastasis of orthotopically transplanted pancreatic tumors in nude mice.
KW - Actin
KW - Amyloid precursor-like protein 2
KW - Metastasis
KW - Migration
KW - Pancreatic cancer
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UR - http://www.scopus.com/inward/citedby.url?scp=84923013443&partnerID=8YFLogxK
U2 - 10.18632/oncotarget.2990
DO - 10.18632/oncotarget.2990
M3 - Article
C2 - 25576918
AN - SCOPUS:84923013443
SN - 1949-2553
VL - 6
SP - 2064
EP - 2075
JO - Oncotarget
JF - Oncotarget
IS - 4
ER -